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公开(公告)号：US20110021768A1
公开(公告)日：2011-01-27
申请号：US12857130
申请日：2010-08-16
IPC分类号： C07H21/04
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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2.发明授权Bioproduction of astaxanthin using mutant carotenoid ketolase and carotenoid hydroxylase genes 失效使用突变类胡萝卜素酮醇酶和类胡萝卜素羟化酶基因生物生产虾青素公开(公告)号：US07074604B1
公开(公告)日：2006-07-11
申请号：US11025177
申请日：2004-12-29
申请人： Xiao-Song Tang , Qiong Cheng , Joanne Y. Shyr , Luan Tao
发明人： Xiao-Song Tang , Qiong Cheng , Joanne Y. Shyr , Luan Tao
CPC分类号： C12N9/0069 , C12N9/0071 , C12P23/00 , Y10S435/858
摘要： Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.
摘要翻译： 提供编码CrtO酮醇酶和CrtZ羟化酶的蛋白质工程化的核酸片段具有增加的虾青素合成活性。 还提供了使用本发明的核酸片段的方法，用于增加或改变合适的生产宿主中的虾青素生产。
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公开(公告)号：US5876945A
公开(公告)日：1999-03-02
申请号：US759581
申请日：1996-12-05
申请人： Dexter Allan Chisholm , Bruce Aaron Diner , Gail K. Donaldson , Howard Paul Hershey , Douglas Brian Jordan , Xiao Song Tang , Shaojie Wang , Jeffrey T. Trost , Patrick V. Warren
发明人： Dexter Allan Chisholm , Bruce Aaron Diner , Gail K. Donaldson , Howard Paul Hershey , Douglas Brian Jordan , Xiao Song Tang , Shaojie Wang , Jeffrey T. Trost , Patrick V. Warren
CPC分类号： C07H21/00 , C12Q1/37 , G01N33/535
摘要： D1 protease has been isolated from the alga (Scenedesmjus obliquus), wheat, and Synechocystis PCC 6803 and the genes encoding these enzymes have been cloned and sequenced. Native or recombinantly produced enzyme has been used to develop assays to detect herbicidal compositions capable of inhibiting the D1 protease enzyme.
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公开(公告)号：US08569469B2
公开(公告)日：2013-10-29
申请号：US12857130
申请日：2010-08-16
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
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公开(公告)号：US07879582B2
公开(公告)日：2011-02-01
申请号：US10374366
申请日：2003-02-26
申请人： Joseph Milano , Xiao-Song Tang
发明人： Joseph Milano , Xiao-Song Tang
CPC分类号： C12N15/1027 , C12N9/88
摘要： A method for the recombination of a gene is disclosed. The method involves the design of unpaired forward and reverse primers having homology to the 5′ end of one template and to the 3′ end of another template. Short primer extension periods results in a recombined template having paired 5′ and 3′ ends that can then be amplified. The amplified sample is devoid of any parental template.
摘要翻译： 公开了一种用于基因重组的方法。 该方法涉及设计具有与一个模板的5'末端和另一个模板的3'末端具有同源性的未配对的正向和反向引物。 短引物延伸期导致具有配对的5'和3'末端的重组模板，然后可以扩增。 扩增的样品没有任何亲本模板。
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公开(公告)号：US07229806B2
公开(公告)日：2007-06-12
申请号：US10439478
申请日：2003-05-16
摘要： An in vivo method for the production of pHS via a recombinant host cell is disclosed. The host cell expresses at least one gene encoding a polypeptide having para-hydroxycinnamic acid decarboxylase activity in combination with either at least one gene encoding a polypeptide having tyrosine ammonia lyase activity or at least one gene encoding a polypeptide having phenylalanine ammonia lyase activity.
摘要翻译： 公开了通过重组宿主细胞产生pHS的体内方法。 宿主细胞表达编码具有对羟基肉桂酸脱羧酶活性的多肽的至少一种基因与至少一种编码具有酪氨酸氨裂解酶活性的多肽的基因或至少一种编码具有苯丙氨酸氨裂解酶活性的多肽的基因的组合。
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公开(公告)号：US20060275881A1
公开(公告)日：2006-12-07
申请号：US11486826
申请日：2006-07-14
申请人： Wei Qi , Sima Sariaslani , Xiao-Song Tang
发明人： Wei Qi , Sima Sariaslani , Xiao-Song Tang
CPC分类号： C12N9/88 , C12P7/40 , C12P7/42 , C12P13/225
摘要： Genes encoding phenylalanine ammonia-lyase (PAL), tyrosine ammonia lyase (TAL) and phenylalanine hydroxylase (PAH) have been introduced into a host organism for the production of Para-hydroxycinnamic acid (PHCA). The introduction of these genes results in the redirection of carbon flow in the host, optimizing the flow of carbon from glucose to PHCA. The intermediates, tyrosine and cinnamic acid are also produced.
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8.发明授权Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide 失效编码突变体Rhodotorula glutinis酪氨酸氨裂解酶多肽的多核苷酸公开(公告)号：US06521748B2
公开(公告)日：2003-02-18
申请号：US09765873
申请日：2001-01-19
申请人： Xiao-Song Tang
发明人： Xiao-Song Tang
IPC分类号： C07H2104
CPC分类号： C12N9/0042 , C12N9/0077 , C12N9/88 , C12N15/8243 , C12P7/42
摘要： The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.
摘要翻译： 本发明提供了几种生物制备对羟基肉桂酸（PHCA）的方法。 本发明还涉及具有将肉桂酸转化为PHCA的能力的新真菌和细菌的发现。 本发明涉及通过将来自酵母红酵母的野生型PAL掺入到大肠杆菌中的葡萄糖转化为PHCA的新的生物催化剂的开发，其强调野生型PAL将酪氨酸转化为PHCA的能力。 本发明还涉及开发新的生物催化剂，用于通过将来自酵母红酵母（Rhodotorula glutinis）加植物细胞色素P-450和细胞色素P-450还原酶的野生型PAL掺入大肠杆菌将葡萄糖转化为PHCA。 在另一个实施方案中，本发明提供了通过具有增强的酪氨酸氨裂解酶（TAL）活性的野生型酵母PAL的诱变来开发新的生物催化剂。
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公开(公告)号：US08445659B2
公开(公告)日：2013-05-21
申请号：US12857120
申请日：2010-08-16
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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公开(公告)号：US20110021767A1
公开(公告)日：2011-01-27
申请号：US12857120
申请日：2010-08-16
IPC分类号： C07H21/04
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
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