发明授权
- 专利标题： Isolated DNA encoding recombinant glucose isomerase
- 专利标题（中）： 分离的DNA编码重组葡萄糖异构酶
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申请号： US12723918申请日： 2010-03-15
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公开(公告)号： US08067561B2公开(公告)日： 2011-11-29
- 发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
- 申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
- 申请人地址： VG Tortola
- 专利权人： BioRight Worldwide Company Limited
- 当前专利权人： BioRight Worldwide Company Limited
- 当前专利权人地址： VG Tortola
- 代理机构： The Webb Law Firm
- 优先权： CN200510123606 20051118
- 主分类号： C12N9/00
- IPC分类号： C12N9/00 ; C12N9/92 ; C12N1/20 ; C12N15/00 ; C07H21/04
摘要：
This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
公开/授权文献
- US20100228016A1 DNA comprising a DNA SEQ. of isolated glucose isomerase 公开/授权日：2010-09-09
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