发明授权
- 专利标题: Method for determining the viability of cells in cell cultures
- 专利标题(中): 确定细胞培养物中细胞活力的方法
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申请号: US12227186申请日: 2007-04-24
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公开(公告)号: US08093015B2公开(公告)日: 2012-01-10
- 发明人: Stefan Obermann , Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria de Jesus , Matthieu Stettler , Martin Jordan
- 申请人: Stefan Obermann , Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria de Jesus , Matthieu Stettler , Martin Jordan
- 申请人地址: DE
- 专利权人: Sartorius Stedim Biotech GmbH
- 当前专利权人: Sartorius Stedim Biotech GmbH
- 当前专利权人地址: DE
- 代理商 Gerald E. Hespos; Michael J. Porco
- 优先权: DE102006022877 20060515
- 国际申请: PCT/EP2007/003568 WO 20070424
- 国际公布: WO2007/131596 WO 20071122
- 主分类号: C12Q1/02
- IPC分类号: C12Q1/02 ; G01N1/18
摘要:
The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
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