发明授权
US08263364B2 Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
有权
确定寡核苷酸和DNA分子核苷酸序列的方法
- 专利标题： Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
- 专利标题（中）： 确定寡核苷酸和DNA分子核苷酸序列的方法
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申请号： US13008303申请日： 2011-01-18
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公开(公告)号： US08263364B2公开(公告)日： 2012-09-11
- 发明人: Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
- 申请人： Peter Williams , Thomas J. Taylor , Daniel J. B. Williams , Ian Gould , Mark A. Hayes
- 申请人地址： US AZ Tempe
- 专利权人： Arizona Board of Regents
- 当前专利权人： Arizona Board of Regents
- 当前专利权人地址： US AZ Tempe
- 代理机构： Brown Rudnick LLP
- 代理商 Thomas Meyers
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C12P1/68 ; C07H21/00
摘要：
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
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