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1.发明授权Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权确定寡核苷酸和DNA分子核苷酸序列的方法公开(公告)号：US08263364B2
公开(公告)日：2012-09-11
申请号：US13008303
申请日：2011-01-18
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中 包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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2.发明申请METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES 有权确定寡核苷酸核苷酸序列和DNA分子的方法公开(公告)号：US20120015353A1
公开(公告)日：2012-01-19
申请号：US13156117
申请日：2011-06-08
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中 包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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3.发明授权Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权确定寡核苷酸和DNA分子核苷酸序列的方法公开(公告)号：US07875440B2
公开(公告)日：2011-01-25
申请号：US11929141
申请日：2007-10-30
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中 包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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公开(公告)号：US09096898B2
公开(公告)日：2015-08-04
申请号：US12969872
申请日：2010-12-16
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
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公开(公告)号：US08263365B2
公开(公告)日：2012-09-11
申请号：US13008392
申请日：2011-01-18
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
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公开(公告)号：US20110183323A1
公开(公告)日：2011-07-28
申请号：US13008392
申请日：2011-01-18
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
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7.发明授权Method of determining the nucleotide sequence of Oligonucleotides and DNA molecules 有权确定寡核苷酸和DNA分子的核苷酸序列的方法公开(公告)号：US08216514B2
公开(公告)日：2012-07-10
申请号：US13156117
申请日：2011-06-08
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中 包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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8.发明授权Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权确定寡核苷酸和DNA分子核苷酸序列的方法公开(公告)号：US07645596B2
公开(公告)日：2010-01-12
申请号：US10709436
申请日：2004-05-05
CPC分类号： C12P19/34 , C12Q1/6816 , C12Q1/6869 , C12Q2565/629 , C12Q2533/101 , C12Q2565/301 , C12Q2563/107 , C12Q2563/103
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA poly-merase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于实时检测将DNA微聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供应）并入到含有 模板系统，其包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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9.发明授权Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权确定寡核苷酸和DNA分子核苷酸序列的方法公开(公告)号：US06780591B2
公开(公告)日：2004-08-24
申请号：US09941882
申请日：2001-08-28
IPC分类号： C12Q168
CPC分类号： C12P19/34 , C12Q1/6816 , C12Q1/6869 , C12Q2565/629 , C12Q2533/101 , C12Q2565/301 , C12Q2563/107 , C12Q2563/103
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中 包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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