发明授权
- 专利标题： Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
- 专利标题（中）： 通过染色质开放剂生产蛋白质的方法和方法，其能使染色质更易于转录因子使用
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申请号： US11156910申请日： 2005-06-20
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公开(公告)号： US08263393B2公开(公告)日： 2012-09-11
- 发明人: Arie Pieter Otte , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
- 申请人： Arie Pieter Otte , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
- 申请人地址： NL Leiden
- 专利权人： Chromagenics B.V.
- 当前专利权人： Chromagenics B.V.
- 当前专利权人地址： NL Leiden
- 代理机构： TraskBritt
- 优先权： EP02080479 20021220; EP03075089 20030113
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N15/63 ; C07H21/04
摘要：
Described are means and methods for providing a cell with a protein expression unit, the method comprising providing a nucleic acid sequence comprising the unit with a nucleic acid sequence encoding a binding site for a member of a chromatin modification system for rendering chromatin more accessible for transcription (opener), wherein the opener is present in the cell. Preferred openers comprise histone modification proteins, chromatin remodeling proteins and trithorax group proteins or equivalents. The cells thus generated and nucleic acid sequences encoding such openers are provided. Openers are preferred in the context of STAR and TRAP sequences.
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