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1.发明申请Expression Augmenting DNA Fragments, Use Thereof, and Methods for Finding Thereof 有权表达增强DNA片段，其用途及其发现方法公开(公告)号：US20100190207A1
公开(公告)日：2010-07-29
申请号：US12225355
申请日：2007-03-20
申请人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
发明人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
CPC分类号： C12N15/63
摘要： The invention provides recombinant DNA molecules comprising novel expression augmenting DNA fragments and an expression cassette, said expression cassette comprising a heterologous promoter linked to a nucleic acid of interest. The invention further provides uses of the novel expression augmenting DNA fragments. The invention further provides methods for obtaining novel expression augmenting DNA fragments.
摘要翻译： 本发明提供重组DNA分子，其包含新增加的表达增加DNA片段和表达盒，所述表达盒包含与感兴趣的核酸连接的异源启动子。 本发明还提供了增加DNA片段的新型表达的用途。 本发明进一步提供了获得新型表达增加DNA片段的方法。
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公开(公告)号：US20090098601A1
公开(公告)日：2009-04-16
申请号：US12226706
申请日：2007-04-24
申请人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
发明人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
CPC分类号： C12N15/67
摘要： The invention provides a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein said DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG startcodon or a TTG startcodon, and wherein the open reading frame sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native open reading frame sequence that encodes the selectable marker protein.
摘要翻译： 本发明提供了包含编码选择性标记多肽的开放阅读框序列的DNA分子，其中编码链中的所述DNA分子包含具有GTG起始密码子或TTG起始密码子的可选择标记多肽的翻译起始序列，并且其中所述开放 与编码可选择标记蛋白的天然开放阅读框序列相比，编码可选择标记蛋白的阅读框序列已被突变以替代其至少一半的CpG二核苷酸。
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3.发明授权Human binding molecules capable of neutralizing influenza virus H3N2 and uses thereof 有权能够中和流感病毒H3N2的人结合分子及其用途公开(公告)号：US08470327B2
公开(公告)日：2013-06-25
申请号：US13138941
申请日：2010-05-06
申请人： Mark Throsby , Robert Heinz Edward Friesen , Theodorus Hendrikus Jacobus Kwaks , Mandy Antonia Catharina Jongeneelen
发明人： Mark Throsby , Robert Heinz Edward Friesen , Theodorus Hendrikus Jacobus Kwaks , Mandy Antonia Catharina Jongeneelen
IPC分类号： A61K39/145 , C12Q1/70 , C07K16/10
CPC分类号： C07K16/1018 , A61K39/12 , A61K2039/505 , A61K2039/507 , A61K2039/545 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/55 , C07K2317/622 , C07K2317/76 , C07K2317/92 , C12N2760/16111
摘要： Binding molecules, such as human monoclonal antibodies, that bind to influenza virus comprising HA of the H3 subtype, such as H3N2, and have a broad neutralizing activity against such influenza virus. Provided are nucleic acid molecules encoding the antibodies, their sequences and compositions comprising the antibodies and methods of identifying or producing the antibodies. The antibodies can be used in the diagnosis, prophylaxis and/or treatment of an influenza virus H3N2 infection. The antibodies may provide cross-subtype protection, such that infections with H3, H7, and/or H10-based influenza subtypes can be prevented and/or treated.
摘要翻译： 结合分子，如人单克隆抗体，其结合包含H3亚型HA的流感病毒，如H3N2，并且具有对这种流感病毒的广泛的中和活性。 提供编码抗体的核酸分子，其序列和包含抗体的组合物以及鉴定或产生抗体的方法。 抗体可用于诊断，预防和/或治疗流感病毒H3N2感染。 抗体可以提供跨亚型保护，使得可以预防和/或治疗H3，H7和/或H10基的流感亚型的感染。
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4.发明授权Expression augmenting DNA fragments, use thereof, and methods for finding thereof 有权表达增加DNA片段，其用途及其发现方法公开(公告)号：US07968700B2
公开(公告)日：2011-06-28
申请号：US12225355
申请日：2007-03-20
申请人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
发明人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
CPC分类号： C12N15/63
摘要： The invention provides recombinant DNA molecules comprising novel expression augmenting DNA fragments and an expression cassette, said expression cassette comprising a heterologous promoter linked to a nucleic acid of interest. The invention further provides uses of the novel expression augmenting DNA fragments. The invention further provides methods for obtaining novel expression augmenting DNA fragments.
摘要翻译： 本发明提供重组DNA分子，其包含新增加的表达增加DNA片段和表达盒，所述表达盒包含与感兴趣的核酸连接的异源启动子。 本发明还提供了增加DNA片段的新型表达的用途。 本发明进一步提供了获得新型表达增加DNA片段的方法。
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5.发明申请HUMAN BINDING MOLECULES CAPABLE OF NEUTRALIZING INFLUENZA VIRUS H3N2 AND USES THEREOF 有权人类结合分子可以中和流感病毒H3N2及其用途公开(公告)号：US20120039898A1
公开(公告)日：2012-02-16
申请号：US13138941
申请日：2010-05-06
申请人： Mark Throsby , Robert Heinz Edward Friesen , Theodorus Hendrikus Jacobus Kwaks , Mandy Antonia Catharina Jongeneelen
发明人： Mark Throsby , Robert Heinz Edward Friesen , Theodorus Hendrikus Jacobus Kwaks , Mandy Antonia Catharina Jongeneelen
IPC分类号： A61K39/395 , C12Q1/70 , A61P31/16 , C12N15/63 , C12N5/10 , C12P21/00 , C07K16/10 , C12N15/12
CPC分类号： C07K16/1018 , A61K39/12 , A61K2039/505 , A61K2039/507 , A61K2039/545 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/55 , C07K2317/622 , C07K2317/76 , C07K2317/92 , C12N2760/16111
摘要： Binding molecules, such as human monoclonal antibodies, that bind to influenza virus comprising HA of the H3 subtype, such as H3N2, and have a broad neutralizing activity against such influenza virus. Provided are nucleic acid molecules encoding the antibodies, their sequences and compositions comprising the antibodies and methods of identifying or producing the antibodies. The antibodies can be used in the diagnosis, prophylaxis and/or treatment of an influenza virus H3N2 infection. The antibodies may provide cross-subtype protection, such that infections with H3, H7, and/or H10-based influenza subtypes can be prevented and/or treated.
摘要翻译： 结合分子，如人单克隆抗体，其结合包含H3亚型HA的流感病毒，如H3N2，并且具有对这种流感病毒的广泛的中和活性。 提供编码抗体的核酸分子，其序列和包含抗体的组合物以及鉴定或产生抗体的方法。 抗体可用于诊断，预防和/或治疗流感病毒H3N2感染。 抗体可以提供跨亚型保护，使得可以预防和/或治疗H3，H7和/或H10基的流感亚型的感染。
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6.发明授权Human binding molecules capable of neutralizing influenza A viruses of phylogenetic group 1 and phylogenetic group 2 and influenza B viruses 有权能够中和系统发育组1和系统发育组2和乙型流感病毒的甲型流感病毒的人结合分子公开(公告)号：US08961978B2
公开(公告)日：2015-02-24
申请号：US14126404
申请日：2012-07-12
申请人： Theodorus Hendrikus Jacobus Kwaks , David Adrianus Theodorus Maria Zuijdgeest , Ronald Vogels , Robert Heinz Edward Friesen
发明人： Theodorus Hendrikus Jacobus Kwaks , David Adrianus Theodorus Maria Zuijdgeest , Ronald Vogels , Robert Heinz Edward Friesen
CPC分类号： C07K16/1018 , A61K39/42 , A61K2039/505 , C07K2317/21 , C07K2317/33 , C07K2317/565 , C07K2317/569 , C07K2317/622 , C07K2317/70 , C07K2317/76 , C07K2317/92 , G01N33/56983 , G01N2333/11
摘要： The present disclosure relates to binding molecules, such as human monoclonal antibodies, that bind to an epitope in the stem region of hemagglutinin of influenza A viruses of phylogenetic group 1 and group 2, as well as influenza B viruses, and have a broad neutralizing activity against such influenza viruses. The disclosure provides nucleic acid molecules encoding the binding molecules, their sequences and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis, prophylaxis and/or treatment of influenza A viruses of phylogenetic groups 1 and 2, as well as influenza B viruses.
摘要翻译： 本公开涉及结合分子系统发生的第1组和第2组甲型流感病毒以及乙型流感病毒血凝素的表位的结合分子，例如人单克隆抗体，并具有广泛的中和活性 针对这种流感病毒。 本公开提供编码结合分子的核酸分子，其序列和包含结合分子的组合物。 结合分子可用于诊断，预防和/或治疗系统发育组1和2的甲型流感病毒以及乙型流感病毒。
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7.发明申请HUMAN BINDING MOLECULES CAPABLE OF NEUTRALIZING INFLUENZA A VIRUSES OF PHYLOGENETIC GROUP 1 AND PHYLOGENETIC GROUP 2 AND INFLUENZA B VIRUSES 有权人类结合分子可以使中性粒细胞病毒1型和生物群2和流感病毒B病毒的病毒中毒公开(公告)号：US20140120113A1
公开(公告)日：2014-05-01
申请号：US14126404
申请日：2012-07-12
申请人： Theodorus Hendrikus Jacobus Kwaks , David Adrianus Theodorus Maria Zuijdgeest , Ronald Vogels , Robert Heinz Edward Friesen
发明人： Theodorus Hendrikus Jacobus Kwaks , David Adrianus Theodorus Maria Zuijdgeest , Ronald Vogels , Robert Heinz Edward Friesen
IPC分类号： C07K16/10
CPC分类号： C07K16/1018 , A61K39/42 , A61K2039/505 , C07K2317/21 , C07K2317/33 , C07K2317/565 , C07K2317/569 , C07K2317/622 , C07K2317/70 , C07K2317/76 , C07K2317/92 , G01N33/56983 , G01N2333/11
摘要： The present disclosure relates to binding molecules, such as human monoclonal antibodies, that bind to an epitope in the stem region of hemagglutinin of influenza A viruses of phylogenetic group 1 and group 2, as well as influenza B viruses, and have a broad neutralizing activity against such influenza viruses. The disclosure provides nucleic acid molecules encoding the binding molecules, their sequences and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis, prophylaxis and/or treatment of influenza A viruses of phylogenetic groups 1 and 2, as well as influenza B viruses.
摘要翻译： 本公开涉及结合分子系统发生的第1组和第2组甲型流感病毒以及乙型流感病毒血凝素的表位的结合分子，例如人单克隆抗体，并具有广泛的中和活性 针对这种流感病毒。 本公开提供编码结合分子的核酸分子，其序列和包含结合分子的组合物。 结合分子可用于诊断，预防和/或治疗系统发育组1和2的甲型流感病毒以及乙型流感病毒。
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公开(公告)号：US07825232B2
公开(公告)日：2010-11-02
申请号：US11632012
申请日：2005-07-07
申请人： Arie Pieter Otte , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt , Henricus Johannes Maria Van Blokland
发明人： Arie Pieter Otte , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt , Henricus Johannes Maria Van Blokland
CPC分类号： C12P21/02 , C07K14/4702 , C12N15/67 , C12N15/79
摘要： The invention provides a novel anti-repressor element, which is useful for improving expression of nucleic acid in host cells. Methods using the novel anti-repressor element for producing a protein of interest are also provided. The invention also provides new configurations of expression cassettes comprising anti-repressor elements.
摘要翻译： 本发明提供了一种新的抗阻遏物，其可用于改善宿主细胞中核酸的表达。 还提供了用于产生感兴趣的蛋白质的新型抗阻遏物元件的方法。 本发明还提供包含抗阻遏物元件的表达盒的新构型。
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9.发明授权Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors 有权通过染色质开放剂生产蛋白质的方法和方法，其能使染色质更易于转录因子使用公开(公告)号：US08263393B2
公开(公告)日：2012-09-11
申请号：US11156910
申请日：2005-06-20
申请人： Arie Pieter Otte , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
发明人： Arie Pieter Otte , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
CPC分类号： C12N15/635 , C12N15/63 , C12N15/85 , C12N2830/48 , C12N2840/203 , C12N2840/206
摘要： Described are means and methods for providing a cell with a protein expression unit, the method comprising providing a nucleic acid sequence comprising the unit with a nucleic acid sequence encoding a binding site for a member of a chromatin modification system for rendering chromatin more accessible for transcription (opener), wherein the opener is present in the cell. Preferred openers comprise histone modification proteins, chromatin remodeling proteins and trithorax group proteins or equivalents. The cells thus generated and nucleic acid sequences encoding such openers are provided. Openers are preferred in the context of STAR and TRAP sequences.
摘要翻译： 描述了用于向细胞提供蛋白质表达单元的方法和方法，所述方法包括提供包含该单元的核酸序列，该核酸序列编码编码染色质修饰系统成员的结合位点的核酸序列，以使得染色质更易于转录 （开环剂），其中开启剂存在于电池中。 优选的开放剂包括组蛋白修饰蛋白，染色质重塑蛋白和trithorax组蛋白或等同物。 提供由此产生的细胞和编码这种开放子的核酸序列。 在STAR和TRAP序列的背景下，开放者是首选的。
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公开(公告)号：US20110300580A1
公开(公告)日：2011-12-08
申请号：US13135966
申请日：2011-07-18
申请人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
发明人： Arie Pieter Otte , Henricus Johannes Maria Van Blokland , Theodorus Hendrikus Jacobus Kwaks , Richard George Antonius Bernardus Sewalt
CPC分类号： C07K14/505 , C12N9/1205 , C12N15/67 , C12N15/85 , C12N2830/46 , C12N2840/20 , C12N2840/203 , C12N2840/206 , C12N2840/50 , C12Y207/01095
摘要： Described is a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein the DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG start codon or a TTG start codon, and wherein the ORF sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native ORF sequence that encodes the selectable marker protein. Further provided are such DNA molecules wherein the ORF sequence that encodes a selectable marker polypeptide is part of a multicistronic transcription unit that further comprises an open reading frame sequence encoding a polypeptide of interest. Also described are methods for obtaining host cells expressing a polypeptide of interest, wherein the host cells comprise the DNA molecules described herein. Further provided is the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules described herein.
摘要翻译： 描述了包含编码可选择标记多肽的开放阅读框序列的DNA分子，其中编码链中的DNA分子包含具有GTG起始密码子或TTG起始密码子的可选择标记多肽的翻译起始序列，其中 与编码可选择标记蛋白的天然ORF序列相比，编码可选择标记蛋白的ORF序列已被突变以替代其至少一半的CpG二核苷酸。 还提供了这样的DNA分子，其中编码可选择标记多肽的ORF序列是多顺反子转录单位的一部分，其进一步包含编码目标多肽的开放阅读框序列。 还描述了获得表达目的多肽的宿主细胞的方法，其中宿主细胞包含本文所述的DNA分子。 还提供感兴趣的多肽的生产，包括培养包含本文所述的DNA分子的宿主细胞。
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