发明授权
- 专利标题: Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm
- 专利标题(中): 使用粒子群优化算法改进重组蛋白表达的序列优化方法
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申请号: US12894401申请日: 2010-09-30
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公开(公告)号: US08326547B2公开(公告)日: 2012-12-04
- 发明人: Xiaowu Liu , Yun He , Zhuying Wang , Chunjiao Wang , Zhibing Liu , Tianhui Xia , Luquan Wang , Fang Liang Zhang
- 申请人: Xiaowu Liu , Yun He , Zhuying Wang , Chunjiao Wang , Zhibing Liu , Tianhui Xia , Luquan Wang , Fang Liang Zhang
- 申请人地址: CN Nanjing
- 专利权人: Nanjingjinsirui Science & Technology Biology Corp.
- 当前专利权人: Nanjingjinsirui Science & Technology Biology Corp.
- 当前专利权人地址: CN Nanjing
- 代理机构: Panitch Schwarze Belisario & Nadel LLP
- 主分类号: G06F19/10
- IPC分类号: G06F19/10 ; G06F19/00
摘要:
An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.