发明授权
- 专利标题: Materials and methods for cryopreserved bone constructs
- 专利标题(中): 低温保存骨构建体的材料和方法
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申请号: US13184357申请日: 2011-07-15
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公开(公告)号: US08367059B2公开(公告)日: 2013-02-05
- 发明人: Teng Ma
- 申请人: Teng Ma
- 申请人地址: US FL Tallahassee
- 专利权人: Florida State University Research Foundation, Inc.
- 当前专利权人: Florida State University Research Foundation, Inc.
- 当前专利权人地址: US FL Tallahassee
- 代理机构: Saliwanchik, Lloyd & Eisenschenk
- 代理商 Doran R. Pace
- 主分类号: A01N63/00
- IPC分类号: A01N63/00 ; C12N11/02 ; C12N5/00
摘要:
Porous HCG scaffolds are provided in a perfusion bioreactor. Cells are seeded in the HCG scaffolds and cell culture media is perfused through the bioreactor to allow for cell seeding and growth. The cell culture media is removed, the HCG-cell constructs washed, and then preserved in the bioreactor with a perfusion comprising cryopreservation fluid comprising one or more of DMSO, trehalose, glycerol, ethylene glycol, or serum. The HCG-cell constructs (or the perfusion chambers containing them) are then removed from the bioreactor and placed in a cryopreservant media and maintained at about −80° C. The frozen HCG-cell constructs (or the chambers containing them) can then be stored at a suitable cryogenic temperature until needed. When needed, frozen HCG-cell constructs can be removed from cold storage and thawed using suitable means.
公开/授权文献
- US20120020934A1 MATERIALS AND METHODS FOR CRYOPRESERVED BONE CONSTRUCTS 公开/授权日:2012-01-26