发明授权
- 专利标题： Expression system for the antibiotic-free production of polypeptides
- 专利标题（中）： 用于无抗生素生产多肽的表达系统
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申请号： US12598712申请日： 2008-02-12
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公开(公告)号： US08465946B2公开(公告)日： 2013-06-18
- 发明人: Céline Cadot , Tina Ploss , Ruth Schwerdtfeger , Bruno Winter
- 申请人： Céline Cadot , Tina Ploss , Ruth Schwerdtfeger , Bruno Winter
- 申请人地址： DE Darmstadt
- 专利权人： AB Enzymes GmbH
- 当前专利权人： AB Enzymes GmbH
- 当前专利权人地址： DE Darmstadt
- 代理机构： Edwards Wildman Palmer LLP
- 代理商 Peter F. Corless; Christine C. O'Day
- 优先权： DE102007021001 20070504
- 国际申请： PCT/EP2008/001977 WO 20080212
- 国际公布： WO2008/135113 WO 20081113
- 主分类号： C12P21/06
- IPC分类号： C12P21/06 ; C12N1/20
摘要：
The present invention relates to a microbial expression system for the production of polypeptides based on the use of extrachromosomal DNA, whereby no antibiotic marker genes for the selection of the host cell but DNA sequences that encode glycerine-3-phosphate dehydrogenase are used, and, thus, the production of the desired polypeptide, e.g., xylanase, does not need the addition of antibiotics. The expression system is free from antibiotic-resistance genes. The invention further relates to a DNA sequence that encodes a polypeptide with glycerine-3-phosphate dehydrogenase activity as well as a polypeptide with glycerine-3-phosphate dehydrogenase activity.