The present invention is directed to a universal serial bus (USB) transaction translator and an associated IN isochronous transaction method. A device interface is coupled to a device via a device bus, and a host interface is coupled to a host via a host bus, wherein the host USB version is higher than the device USB version. At least two buffers configured to store data are disposed between the device interface and the host interface. A controller stores the data in the buffers alternately. A register is used to record device bus information. Before the host sends an IN packet, the controller pre-fetches data from the device according to the device bus information and then stores the data in the buffers; the controller responds with the pre-fetched data to the host after the host sends the IN packet.