A universal memory I/O generating apparatus includes a defining module, a retrieving module, a generating module, and a layout module. The defining module defines a mapping table according to a pin configuration of a plurality of I/Os. The mapping table includes corresponding relationships between the plurality of IOs and a plurality of memory functions. The retrieving module retrieves control information corresponding to the mapping table from candidate information, which is associated with the corresponding relationships between the plurality of I/Os and the plurality of memory functions. The generating module generates a hardware description language (HDL) file according to the control information. The layout module programs the plurality of I/Os according to the HDL file, so that each of the I/Os can correspond to its corresponding memory function.