A disposable biosensor test strip is provided that includes a plurality of penetrating members. Each penetrating member is associated with a capillary chamber that has a depth suitable for capillary flow of blood and holds a volume of less than about 1.0 .μl of the blood sample. A working electrode and a counter or reference electrode are disposed within the capillary chamber. A reagent is proximal to or in contact with at least the working electrode. The reagent includes an enzyme and a mediator. The reagent reacts with glucose to produce an electroactive reaction product. A blood sample, containing glucose, is applied into the capillary chamber. The capillary chamber directs capillary flow of the blood sample into contact with the reagent to cause the blood sample to at least partially solubilize or hydrate the reagent. The blood sample is detected in the capillary chamber. The electroactive reaction product is electro-oxidized or electro-reduced at the working electrode. Within 10 seconds after detecting, a determination is made of glucose concentration and a readout of the measurement is provided. The glucose determination is made by correlating the electro-oxidized or electro-reduced electroactive reaction product to the concentration of glucose in the blood sample.