Devices and methods for detecting an analyte are provided. Devices for voltage sensing of analytes may comprise a fluidic channel defined in a substrate, a pair of sensing electrodes disposed in a fluidic channel for sensing voltage therein, and a pair of electromotive electrodes for applying potential along the fluidic channel. The pair of sensing electrodes may include a first and second sensing electrode disposed at two discrete locations along the length of the fluidic channel and the pair of electromotive electrodes may be disposed at a first end and a second end of the fluidic channel. The fluidic channel may include a nanochannel or a microchannel. Methods for detecting an analyte may include the steps of disposing the analyte in a fluidic channel; applying a potential along the fluidic channel to generate an electrophoretic force therein such that the analyte is translocated from a first end of the fluidic channel to a second end of the fluidic channel; and measuring a voltage signal between a pair of sensing electrodes disposed in the fluidic channel as the analyte moves past the sensing electrodes.