发明授权
US08936925B2 Reconstitution of 5-enolpyruvylshikimate-3-phosphate synthase activity by fragment complementation
有权
通过片段互补重建5-烯醇丙酮酸莽草酸-3-磷酸合酶活性
- 专利标题： Reconstitution of 5-enolpyruvylshikimate-3-phosphate synthase activity by fragment complementation
- 专利标题（中）： 通过片段互补重建5-烯醇丙酮酸莽草酸-3-磷酸合酶活性
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申请号： US11721856申请日： 2005-12-16
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公开(公告)号： US08936925B2公开(公告)日： 2015-01-20
- 发明人: Yiping Wang , Yicheng Sun , Yan Li
- 申请人： Yiping Wang , Yicheng Sun , Yan Li
- 申请人地址： CN Beijing
- 专利权人： Peking University
- 当前专利权人： Peking University
- 当前专利权人地址： CN Beijing
- 代理机构： RatnerPrestia
- 优先权： CN200410102289 20041216
- 国际申请： PCT/CN2005/002219 WO 20051216
- 国际公布： WO2006/063533 WO 20060622
- 主分类号： C12N9/10
- IPC分类号： C12N9/10 ; C12N15/82
摘要：
The present invention relates to protein fragments of 5-enolpyruvylshikimate-3-phosphate synthase, which are selected from the protein fragment pairs of EPSPS, two such protein fragments can make up full length EPSPS and reconstitute EPSPS activities by complementation without help of any joint structure. The present invention also relates to nucleic acid molecules encoding the protein fragments, expression vectors and cells comprising such nucleic acid molecules. The present invention also relates to methods for reconstituting EPSPS activities by using the fragments or the nucleic acid molecules or the expression vectors of the present invention, as well as methods for dividing the protein fragments of the present invention.
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