发明授权
- 专利标题： Production host cell lines
- 专利标题（中）： 生产宿主细胞系
-
申请号： US12507252申请日： 2009-07-22
-
公开(公告)号： US08962312B2公开(公告)日： 2015-02-24
- 发明人: Hitto Kaufmann , Lore Florin , Eric Becker , Joey M. Studts
- 申请人： Hitto Kaufmann , Lore Florin , Eric Becker , Joey M. Studts
- 申请人地址： DE Ingelheim
- 专利权人： Boehringer Ingelheim Pharma GmbH & Co. KG
- 当前专利权人： Boehringer Ingelheim Pharma GmbH & Co. KG
- 当前专利权人地址： DE Ingelheim
- 代理商 Michael P. Morris; Edouard G. Lebel; Usha R. Patel
- 优先权： EP08161030 20080723; EP08164054 20080910
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; C12P21/02 ; C07K16/00 ; C12N9/06 ; C12N15/63
摘要：
The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpper® Discovery) and DSM ACC2910 (CHOpper® Standard). The invention further concerns a method of producing proteins using the cells generated by the described method.
公开/授权文献
- US20100021911A1 PRODUCTION HOST CELL LINES 公开/授权日：2010-01-28
信息查询
IPC分类:
