Invention Grant
- Patent Title: Organisms for the production of cyclohexanone
- Patent Title (中): 用于生产环己酮的生物体
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Application No.: US14172079Application Date: 2014-02-04
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Publication No.: US09139853B2Publication Date: 2015-09-22
- Inventor: Robin E. Osterhout , Anthony P. Burgard , Mark J. Burk , Priti Pharkya
- Applicant: Genomatica, Inc.
- Applicant Address: US CA San Diego
- Assignee: Genomatica, Inc.
- Current Assignee: Genomatica, Inc.
- Current Assignee Address: US CA San Diego
- Agent Jones Day
- Main IPC: C12N9/16
- IPC: C12N9/16 ; C12N9/88 ; C12N1/20 ; C12P7/26 ; C12N9/00 ; C12N15/52 ; C12N15/70

Abstract:
A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.
Public/Granted literature
- US20140356919A1 ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE Public/Granted day:2014-12-04
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