发明授权
- 专利标题: Method for single cell genome analysis and kit therefor
- 专利标题(中): 单细胞基因组分析方法及其试剂盒
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申请号: US13976845申请日: 2011-12-29
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公开(公告)号: US09238840B2公开(公告)日: 2016-01-19
- 发明人: Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
- 申请人: Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
- 申请人地址: CN Shenzhen
- 专利权人: BGI-Shenzhen Co., Limited
- 当前专利权人: BGI-Shenzhen Co., Limited
- 当前专利权人地址: CN Shenzhen
- 代理机构: Drinker Biddle & Reath LLP
- 优先权: CN201010619689 20101231
- 国际申请: PCT/CN2011/084959 WO 20111229
- 国际公布: WO2012/089148 WO 20120705
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.
公开/授权文献
- US20140017683A1 METHOD FOR SINGLE CELL GENOME ANALYSIS AND KIT THEREFOR 公开/授权日:2014-01-16
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