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公开(公告)号:US09238840B2
公开(公告)日:2016-01-19
申请号:US13976845
申请日:2011-12-29
申请人: Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
发明人: Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6888 , C12Q1/6806 , C12Q1/6851 , C12Q1/6869 , C12Q1/6876 , C12Q2600/166 , C12Q2531/119 , C12Q2545/101
摘要: A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.
摘要翻译: 提供了用于分析单细胞的基因组的方法,并且还提供了试剂盒。 用于分析单细胞基因组的方法可以包括分离和裂解单细胞以获得细胞的全基因组DNA; 对全基因组DNA进行全基因组扩增以获得全基因组扩增产物; 使用全基因组扩增产物作为模板进行PCR扩增,并使用管家基因特异性引物检测全基因组扩增产物的管家基因; 并且基于检测结果确定全基因组扩增产物是否满足测序要求,其中扩增产物在每个染色体中的均匀分布是满足测序要求的扩增产物的指示。
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公开(公告)号:US20140017683A1
公开(公告)日:2014-01-16
申请号:US13976845
申请日:2011-12-29
申请人: Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
发明人: Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6888 , C12Q1/6806 , C12Q1/6851 , C12Q1/6869 , C12Q1/6876 , C12Q2600/166 , C12Q2531/119 , C12Q2545/101
摘要: A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.
摘要翻译: 提供了用于分析单细胞的基因组的方法,并且还提供了试剂盒。 用于分析单细胞基因组的方法可以包括分离和裂解单细胞以获得细胞的全基因组DNA; 对全基因组DNA进行全基因组扩增以获得全基因组扩增产物; 使用全基因组扩增产物作为模板进行PCR扩增,并使用管家基因特异性引物检测全基因组扩增产物的管家基因; 并且基于检测结果确定全基因组扩增产物是否满足测序要求,其中扩增产物在每个染色体中的均匀分布是满足测序要求的扩增产物的指示。
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