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    Method for single cell genome analysis and kit therefor
    1.
    发明授权
    Method for single cell genome analysis and kit therefor 有权
    单细胞基因组分析方法及其试剂盒

    公开(公告)号：US09238840B2

    公开(公告)日：2016-01-19

    申请号：US13976845

    申请日：2011-12-29

    申请人： Xuyang Yin ,  Li Bao ,  Xun Xu ,  Hanjie Wu ,  Xiaoyu Liu ,  Xiuqing Zhang ,  Huanming Yang

    发明人： Xuyang Yin ,  Li Bao ,  Xun Xu ,  Hanjie Wu ,  Xiaoyu Liu ,  Xiuqing Zhang ,  Huanming Yang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6888 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2600/166 ,  C12Q2531/119 ,  C12Q2545/101

    摘要： A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.

    摘要翻译： 提供了用于分析单细胞的基因组的方法，并且还提供了试剂盒。 用于分析单细胞基因组的方法可以包括分离和裂解单细胞以获得细胞的全基因组DNA; 对全基因组DNA进行全基因组扩增以获得全基因组扩增产物; 使用全基因组扩增产物作为模板进行PCR扩增，并使用管家基因特异性引物检测全基因组扩增产物的管家基因; 并且基于检测结果确定全基因组扩增产物是否满足测序要求，其中扩增产物在每个染色体中的均匀分布是满足测序要求的扩增产物的指示。

    METHOD FOR SINGLE CELL GENOME ANALYSIS AND KIT THEREFOR
    2.
    发明申请
    METHOD FOR SINGLE CELL GENOME ANALYSIS AND KIT THEREFOR 有权
    单细胞基因组分析方法及其制备方法

    公开(公告)号：US20140017683A1

    公开(公告)日：2014-01-16

    申请号：US13976845

    申请日：2011-12-29

    申请人： Xuyang Yin ,  Li Bao ,  Xun Xu ,  Hanjie Wu ,  Xiaoyu Liu ,  Xiuqing Zhang ,  Huanming Yang

    发明人： Xuyang Yin ,  Li Bao ,  Xun Xu ,  Hanjie Wu ,  Xiaoyu Liu ,  Xiuqing Zhang ,  Huanming Yang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6888 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2600/166 ,  C12Q2531/119 ,  C12Q2545/101

    摘要： A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.

    摘要翻译： 提供了用于分析单细胞的基因组的方法，并且还提供了试剂盒。 用于分析单细胞基因组的方法可以包括分离和裂解单细胞以获得细胞的全基因组DNA; 对全基因组DNA进行全基因组扩增以获得全基因组扩增产物; 使用全基因组扩增产物作为模板进行PCR扩增，并使用管家基因特异性引物检测全基因组扩增产物的管家基因; 并且基于检测结果确定全基因组扩增产物是否满足测序要求，其中扩增产物在每个染色体中的均匀分布是满足测序要求的扩增产物的指示。

    SINGLE CELL CLASSIFICATION METHOD, GENE SCREENING METHOD AND DEVICE THEREOF
    3.
    发明申请
    SINGLE CELL CLASSIFICATION METHOD, GENE SCREENING METHOD AND DEVICE THEREOF 审中-公开
    单细胞分类方法，基因筛选方法及其装置

    公开(公告)号：US20140206006A1

    公开(公告)日：2014-07-24

    申请号：US14239650

    申请日：2012-08-24

    申请人： Xun Xu ,  Li Bao ,  Weiming He ,  Yong Hou ,  Ye Tao

    发明人： Xun Xu ,  Li Bao ,  Weiming He ,  Yong Hou ,  Ye Tao

    IPC分类号： G06F19/22

    CPC分类号： G16B30/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  G16B20/00

    摘要： Provided are a single cell classification method, a gene screening method and a device for implementing the method. In that, the single cell classification method includes the following steps: sequencing the whole genomes of a plurality of single cell samples from the same group, respectively, so as to obtain reads from each single cell sample; aligning the reads from each single cell sample to the sequence of a reference genome, respectively, and performing data filtering on said reads; on the basis of the filtered reads, determining a consistent genotype of each single cell sample, in which consistent genotypes of all the single cell samples constitute an SNP dataset of said group; aimed at said each single cell, on the basis of the SNP dataset of said group, determining a corresponding genotype for each cell at a site corresponding to a position in an SNP dataset of the reference genome; and selecting an SNP site associated with cell mutation, and on the basis of the genotype of said single cell at the site, classifying said single cell.

    摘要翻译： 提供单细胞分类方法，基因筛选方法和实施该方法的装置。 其中，单细胞分类方法包括以下步骤：从同一组分别测序多个单细胞样品的全基因组，从而获得每个单细胞样品的读数; 将每个单个细胞样本的读数分别对准参考基因组的序列，并对所述读取执行数据过滤; 基于经过滤的读数，确定每个单细胞样品的一致的基因型，其中所有单细胞样品的一致基因型构成所述组的SNP数据集; 针对所述每个单细胞，基于所述组的SNP数据集，在对应于参照基因组的SNP数据集中的位置的位点处确定每个细胞的相应基因型; 并选择与细胞突变相关的SNP位点，并且基于所述单细胞在所述位点处的基因型，对所述单细胞进行分类。

    User Programable Touch and Motion Controller
    4.
    发明申请
    User Programable Touch and Motion Controller 审中-公开
    用户可编程触摸和运动控制器

    公开(公告)号：US20160139628A1

    公开(公告)日：2016-05-19

    申请号：US14940787

    申请日：2015-11-13

    申请人： Li Bao ,  Lyle Philip Arenson ,  Jonathan Schroeder

    发明人： Li Bao ,  Lyle Philip Arenson ,  Jonathan Schroeder

    IPC分类号： G06F1/16 ,  G06F3/16 ,  G06F3/0488 ,  G06F3/0482 ,  G06F3/041 ,  G06F3/0346

    CPC分类号： G06F1/163 ,  G06F1/1643 ,  G06F3/017 ,  G06F3/0346 ,  G06F3/0482 ,  G06F3/04847 ,  G06F3/04883 ,  G06F2203/04808

    摘要： A wearable device having a display and a touchscreen is disclosed. The device is capable of interfacing with and controlling remotely controllable devices. The device is capable of sensing swipes, touches, and gestures, which are mapped to custom command and control functions. Swipes and touches may be with one finger or multiple fingers simultaneously, Gestures may be any motion limited only by the sensitivity of the motion sensing devices. In alternative embodiments, the device further consists of a speaker and microphone to allow spoken commands to be used in conjunction with, or replace, swipe, touch, and gestures to enhance the efficiency or ease of use of the device. In other alternative embodiments, the device may connect to a remote controllable device through a cloud, internet, or cellular network.

    摘要翻译： 公开了一种具有显示器和触摸屏的可穿戴设备。 该设备能够与远程可控设备进行接口和控制。 该设备能够检测到映射到自定义命令和控制功能的滑动，触摸和手势。 滑动和触摸可以同时用一个手指或多个手指，手势可以是仅由运动感测装置的灵敏度限制的任何运动。 在替代实施例中，设备还包括扬声器和麦克风，以允许口语命令与滑动，触摸和手势结合使用或替换，以提高设备的效率或易用性。 在其他替代实施例中，设备可以通过云，互联网或蜂窝网络连接到远程可控设备。

    FILTER MEDIUM FOR AIR FILTER AND AIR FILTER
    5.
    发明申请
    FILTER MEDIUM FOR AIR FILTER AND AIR FILTER 审中-公开
    用于空气过滤器和空气过滤器的过滤介质

    公开(公告)号：US20100192531A1

    公开(公告)日：2010-08-05

    申请号：US12733547

    申请日：2008-03-31

    申请人： Li Bao ,  Makoto Kobayashi ,  Shiro Hayashi ,  Jincheol Kim ,  Hitoshi Niinuma

    发明人： Li Bao ,  Makoto Kobayashi ,  Shiro Hayashi ,  Jincheol Kim ,  Hitoshi Niinuma

    IPC分类号： B01D46/52 ,  B01D39/14

    CPC分类号： B01D46/546 ,  B01D39/1623 ,  B01D39/1638 ,  D01D5/0038 ,  D04H1/728

    摘要： An air filter comprising a filter pack comprising a folded nanofiber filter medium, fitted to a filter frame through a seal material, the nanofiber filter medium being a filter medium for an air filter, comprising a nanofiber filter medium which, when a filter medium face velocity is 5.3 cm/s, has a performance index of collecting target particle diameter of 0.3 μm, 0.040 (1/Pa) or more, performance index of collecting target particle diameter of 0.1 μm, 0.030 (1/Pa) or more, and performance index of collecting target particle diameter of 0.05 μm, 0.050 (1/Pa) or more. By this, a high air volume and low pressure drop type high-performance air filter using a nanofiber filter medium can be provided.

    摘要翻译： 一种空气过滤器，包括一个过滤组件，该过滤器组件包括折叠的纳米纤维过滤介质，通过密封材料装配到过滤器框架上，该纳米纤维过滤介质是用于空气过滤器的过滤介质，包括纳滤纤维过滤介质，当过滤介质面向速度 为5.3cm / s，具有0.3μm的收集目标粒径，0.040（1 / Pa）以上的收集目标粒径的性能指标，0.1μm的收集目标粒径的性能指标，0.030（1 / Pa）以上，性能指标 收集目标粒径为0.05μm，0.050（1 / Pa）以上的指标。 由此，可以提供使用纳米纤维过滤介质的高风量和低压降型高性能空气过滤器。