利索-全球专利检索

  1. 登录
  2. 注册
发明授权
US09290770B2 Coryneform bacterium transformant and process for producing valine using the same 有权
棒状细菌转化体和使用其制备缬氨酸的方法

Coryneform bacterium transformant and process for producing valine using the same
摘要：
A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host.(a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof.(b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.
摘要（中）：

将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。 （a）编码源自谷氨酸棒杆菌的乙酰羟酸合酶的DNA，其在DNA编码的氨基酸序列或其类似物中具有将位置156处的甘氨酸变为谷氨酸（G156E）的突变。 （b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将第34位的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸 （R49F），其由DNA编码的氨基酸序列或其类似物。 （c）编码源自嗜裂杆菌属的亮氨酸脱氢酶的DNA，或其类似物。

公开/授权文献
信息查询
Espacenet
0/0