A reagent delivery device includes a reagent delivery column with a housing receiving reagent storage elements that can move, and a breaching element coupled to the housing. The device includes an actuation member that during operation forces the reagent storage elements toward the breaching element. Breaching the storage elements releases the reagent. The breaching element and/or the housing are configured to communicate reagent between the reagent storage element and a target chamber coupled to the housing. The breaching element can be a needle, a blade, or a combination thereof. Multiple reagent delivery columns can be coupled to microplate wells for larger scale reagent delivery and processing. Biasing elements such as a spring and a counterweight can be directly or indirectly coupled to the housing and/or the reagent storage elements, resisting and/or moderating movement of the reagent elements.