A semiconductor substrate may be formed by providing an providing a semiconductor-on-insulator (SOI) substrate including a base semiconductor layer, a buried insulator layer above the base semiconductor layer, and a SOI layer comprising a first semiconductor material above the buried insulator layer; forming an isolation region in the SOI layer isolating a first portion of the SOI layer from a second portion of the SOI layer; removing the second portion of the SOI layer to expose a portion of the buried insulator layer; forming a hole in the exposed portion of the buried insulator layer to expose a portion of the base semiconductor layer; and forming a semiconductor layer made of a second semiconductor material on the exposed portion of the base semiconductor layer, so that the replacement semiconductor layer covers the exposed region of the buried insulator layer.