The present disclosure provides an automatic locking screw machine, which includes an automatic separating module, an automatic feeding module, and an automatic locking module. The automatic separating module separates a plurality of screws arranged in an array. The automatic feeding module is connected to the automatic separating module, and includes a feeding duct for conveying the screws separated by the automatic separating module. The automatic locking module is connected to the automatic feeding module, and includes a first spring ball mechanism and a screwdriver. The first spring ball mechanism supports the screws conveyed by the feeding duct, and the screwdriver locks the screws supported by the first spring ball mechanism onto a workpiece.