Invention Grant
- Patent Title: Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
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Application No.: US13781318Application Date: 2013-02-28
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Publication No.: US09506845B2Publication Date: 2016-11-29
- Inventor: Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A. A. West
- Applicant: Fluidigm Corporation
- Applicant Address: US CA South San Francisco
- Assignee: Fluidigm Corporation
- Current Assignee: Fluidigm Corporation
- Current Assignee Address: US CA South San Francisco
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12P19/34
- IPC: C12P19/34 ; G01N1/28 ; C12Q1/68 ; G01N1/34 ; G01N15/14 ; B01L3/00 ; B01L7/00

Abstract:
Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
Public/Granted literature
- US20130296196A1 METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS Public/Granted day:2013-11-07
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