发明授权
- 专利标题： Reverse transcriptase having improved thermostability
- 专利标题（中）： 逆转录酶具有改善的热稳定性
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申请号： US13984746申请日： 2012-02-07
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公开(公告)号： US09534210B2公开(公告)日： 2017-01-03
- 发明人: Han Oh Park , Sung Jun Yang , Sung Mo Joo , Byoung Oh Hwang
- 申请人： Han Oh Park , Sung Jun Yang , Sung Mo Joo , Byoung Oh Hwang
- 申请人地址： KR Daejeon
- 专利权人： BIONEER CORPORATION
- 当前专利权人： BIONEER CORPORATION
- 当前专利权人地址： KR Daejeon
- 代理机构： Rothwell, Figg, Ernst & Manbeck, P.C.
- 优先权： KR10-2011-0011639 20110209
- 国际申请： PCT/KR2012/000894 WO 20120207
- 国际公布： WO2012/108672 WO 20120816
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N1/20 ; C12N15/70 ; C12P19/34
摘要：
The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.
公开/授权文献
- US20140045244A1 REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY 公开/授权日：2014-02-13
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