公开(公告)号：US20220074898A1

公开(公告)日：2022-03-10

申请号：US17309868

申请日：2019-10-24

申请人： BIONEER CORPORATION

发明人： Han-Oh PARK ,  Taeman KIM ,  Jong Rok AHN ,  Jae-Ha KIM

IPC分类号： G01N30/30 ,  G01N30/60 ,  H05B3/14

摘要： A heater integrated gas chromatography (GC) column device according to the present invention is capable of precisely and uniformly controlling a temperature by having a high thermal conductivity, and raising and lowering a temperature at a high speed by having a low thermal mass, such that a measuring time is significantly decreased. The GC column is in contact with a bobbin with a homogeneous temperature distribution, and thus a temperature is homogeneously distributed in each GC column. Further, the heater integrated GC column device according to the present invention has the above-described effects and may have a smaller size.

公开(公告)号：US20210369758A1

公开(公告)日：2021-12-02

申请号：US17402081

申请日：2021-08-13

申请人： BIONEER CORPORATION

发明人： Taewoo LEE ,  Sanghyung Shim ,  Ungsik Yu ,  Han-Oh Park

IPC分类号： A61K31/713 ,  A61P35/00 ,  A61K31/25 ,  C12N15/113

摘要： The present invention relates to a double-stranded oligo RNA structure comprising double-stranded miRNA, and a composition for preventing or treating cancer, containing the same. More specifically, the present invention relates to an anti-cancer pharmaceutical composition, containing: a double-stranded oligo RNA structure comprising miR-3670, miR-4477a and miR-8078, and characterized by a method for effectively inhibiting cancer cell proliferation or inducing cancer cell apoptosis; and a pharmaceutically acceptable carrier.

公开(公告)号：US20210233759A1

公开(公告)日：2021-07-29

申请号：US15734848

申请日：2019-06-04

申请人： BIONEER CORPORATION

发明人： Taeman KIM ,  Han-Oh PARK

IPC分类号： H01J49/06 ,  H01J49/24 ,  H01J49/16

摘要： An ion guide for transferring ions to a mass analyzer is disclosed. The ion guide is characterized in that a plurality of DC rings and a plurality of RF multi-pole rings with a plurality of electrodes are arranged alternatingly along a central axis between an inlet into which the ions move and an outlet for transferring the ions. The inner diameter of the DC rings is kept constant and the inner diameter of the RF multi-pole rings gradually decreases in a direction from the inlet to the outlet.

公开(公告)号：US20210032628A1

公开(公告)日：2021-02-04

申请号：US16963998

申请日：2019-01-29

申请人： BIONEER CORPORATION

发明人： Taewoo LEE ,  Jiwon RYU ,  Eun Ji IM

IPC分类号： C12N15/113 ,  A61P35/00

摘要： The present invention relates to a double-helix oligonucleotide construct comprising a double-stranded miRNA and a composition for preventing or treating cancer comprising the same. More particularly, the present invention relates to a double-helix oligonucleotide construct comprising miR-544a characterized by a method that effectively inhibits the proliferation of cancer cells or induces a voluntary death of cancer cells, and an anticancer composition comprising the construct.

公开(公告)号：US10441957B2

公开(公告)日：2019-10-15

申请号：US15103102

申请日：2014-12-05

申请人： BIONEER CORPORATION

发明人： Han Oh Park ,  Jong Kab Kim

IPC分类号： B03C1/01 ,  C12N15/10 ,  G01N1/40 ,  B03C1/28 ,  B03C1/033

摘要： The present invention relates to a magnetic particle separating device, and a method of separating and purifying nucleic acid or protein using the same. The device comprises: induction magnets (100); an induction magnet fixing part (200) having induction magnet fixing holes (210) for fixing the induction magnets (100); and a body (300) in which entry holes (310) are formed into which tubes (T) are inserted. Thus, the application and removal of a magnetic field to and from the body is made very convenient, so that the device can be very advantageously used in the separation and purification of nucleic acid or protein.

公开(公告)号：US10287542B2

公开(公告)日：2019-05-14

申请号：US14648891

申请日：2013-11-29

申请人： BIONEER CORPORATION

发明人： Han Oh Park ,  Jong Kab Kim ,  Ji Won Han ,  You Sang Cho ,  Min Jung Kim ,  Ha Neul Kim ,  Yang Won Lee ,  Nam Il Kim

IPC分类号： C07K1/14 ,  C12M1/00 ,  C12M1/24 ,  C12M1/32 ,  C12M1/26 ,  C12M1/34 ,  C12P21/00 ,  C07K1/02 ,  C07K1/04 ,  C07K1/22 ,  C07K1/36

摘要： An automated cell-free protein production system comprises: a protein expression reaction unit comprising a reaction vessel that includes a plurality of dialysis tubes, each including a dialysis membrane and being open at its top; a reaction temperature control unit configured to heat or cool the reaction vessel; a pipette array comprising a plurality of pipettes and configured to suck or discharge solutions using the pipettes; a pipette array moving unit configured to move the pipette array in an upward and downward direction, a forward and backward direction or a left and right direction so as to move solutions; a protein purification unit including a magnetic field application device; and a multi-well plate mounting unit having mounted therein a multi-well plate kit configured to supply solutions that are used for protein production.

公开(公告)号：US10022717B2

公开(公告)日：2018-07-17

申请号：US14875246

申请日：2015-10-05

申请人： BIONEER CORPORATION

发明人： Han Oh Park ,  Gu Young Song ,  Jung A Bae

IPC分类号： B01L1/00 ,  B01L3/00 ,  G01N21/64 ,  C12M1/32

摘要： A method of manufacturing a micro-chamber plate with a built-in sample, including: settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening; disposing a cover for micro-chamber plate receiving part to cover the upper opening, the cover for micro-chamber plate receiving part having a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole for auxiliary covering part; and manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate through a vessel communication part which is formed at the provisional storing part to be communicated with the micro-chamber plate receiving part.

公开(公告)号：USRE46912E1

公开(公告)日：2018-06-26

申请号：US14537565

申请日：2014-11-10

申请人： Bioneer Corporation

发明人： Ji Hee Kang ,  Byeung I. You ,  Sung I. Yun ,  Han O. Park

IPC分类号： A61K35/74 ,  C12N1/20 ,  C12R1/225 ,  A23L33/18 ,  A23L33/135 ,  A23C9/123 ,  C07K14/335 ,  A61K38/00

CPC分类号： C12R1/225 ,  A23C9/1234 ,  A23L33/135 ,  A23L33/18 ,  A23Y2220/37 ,  A61K38/00 ,  C07K14/335 ,  C12N1/20

摘要： The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well. Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.

公开(公告)号：US09649388B2

公开(公告)日：2017-05-16

申请号：US14372211

申请日：2013-01-17

申请人： BIONEER CORPORATION

发明人： Jeiwook Chae ,  Hyunjin Chung ,  Boreum Lee ,  Han Oh Park

IPC分类号： A61K47/48 ,  C12N15/11 ,  A61K49/18 ,  A61K9/14 ,  A61K31/7105 ,  A61K9/51 ,  A61K31/713 ,  A61K33/24 ,  A61K9/50

CPC分类号： A61K47/48015 ,  A61K9/14 ,  A61K9/5094 ,  A61K9/5115 ,  A61K31/7105 ,  A61K31/713 ,  A61K33/24 ,  A61K47/6923 ,  A61K49/1857 ,  C12N15/11 ,  A61K2300/00

摘要： Provided are a SAMiRNA-magnetic nanoparticle complex capable of effectively delivering a double-stranded oligo RNA and magnetic nanoparticles into a cell and a composition capable of simultaneously performing diagnosis and therapy of diseases such as cancer, and the like, containing the same. More specifically, provided is the SAMiRNA-magnetic nanoparticle complex consisting of double-stranded oligo RNA-polymer structures in which a hydrophilic material and a second hydrophobic material are bound to the double-stranded oligo RNA by a simple covalent bond or a linker-mediated covalent bond, and the magnetic nanoparticles in which a first hydrophobic material is bound onto a surface of the magnetic material, as a core.The SAMiRNA-magnetic nanoparticle complex may have a homogeneous size by a hydrophobic interaction between the first hydrophobic material of the present invention and the second hydrophobic material of the double-stranded oligo RNA structure.In addition, the hydrophilic material and the second hydrophobic material bound to the double-stranded oligo RNA structure may improve in vivo stability of the double-stranded oligo RNA, an additionally bound ligand may deliver the SAMiRNA-magnetic nanoparticle complex into a target cell even at a relative low concentration of dosage, and the magnetic materials of the magnetic nanoparticles may be used as an imaging agent for diagnosis.

公开(公告)号：US20160145622A1

公开(公告)日：2016-05-26

申请号：US14902564

申请日：2014-07-04

申请人： BIONEER CORPORATION

发明人： Joo Sung Yang ,  Woo Seok Kim ,  Soon Ja Choi ,  Han Oh Park

IPC分类号： C12N15/113

CPC分类号： C12N15/1131 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2310/3521 ,  C12N2310/3525 ,  C12N2310/3533

摘要： The present invention relates to a dengue virus-specific siRNA, a double-stranded oligo RNA structure comprising the siRNA, and a composition for inhibiting dengue virus replication, which comprises the same, in which the double-stranded oligo RNA structure comprises a hydrophilic compound and hydrophobic compound conjugated to both ends of the double-stranded RNA (siRNA) by a single covalent bond or a linker-mediated covalent bond so that they will be efficiently delivered into cells, and can be converted into nanoparticles by hydrophobic interactions between the double-stranded oligo RNA structures in an aqueous solution. The siRNA included in the double-stranded oligo RNA structure acts specifically on all dengue virus serotypes. The present invention also relates to a method for preparing the double-stranded oligo RNA structure, and a pharmaceutical composition for preventing or treating dengue virus infection, which comprises the double-stranded oligo RNA structure.

摘要翻译： 本发明涉及登革病毒特异性siRNA，包含siRNA的双链寡聚RNA结构和用于抑制登革病毒复制的组合物，其包含其中双链寡RNA结构包含亲水化合物 和通过单个共价键或连接体介导的共价键与双链RNA（siRNA）的两端缀合的疏水性化合物，使得它们将被有效地递送到细胞中，并且可以通过双重的疏水相互作用转化成纳米颗粒 在水溶液中的品系寡聚RNA结构。 包含在双链寡聚RNA结构中的siRNA特异性地对所有登革热病毒血清型起作用。 本发明还涉及制备双链寡聚RNA结构的方法，以及包含双链寡聚RNA结构的用于预防或治疗登革病毒感染的药物组合物。