Disclosed are a method and system for determining and controlling power of an optical transmitter of an optical network unit (ONU) for a time and wavelength division multiplexing passive optical network (TWDM-PON). The system includes an RSSI collector configured to collect received signal strength indication (RSSI) information from upstream optical signals received from the ONUs connected to optical line terminal (OLT) ports, an ONU power level determiner configured to gather the pieces of RSSI information about the ONUs from the RSSI collector, and to determine power of optical transmitters of the ONUs based on the gathered information, and a power mode controller configured to receive power mode setting information of the optical transmitters of the ONUs from the ONU power level determiner, and to generate a physical layer operation and maintenance (PLOAM) message to control power modes of the ONUs based on the received power mode setting information.