发明授权
- 专利标题: In vitro reconstituted plant virus capsids for delivering RNA genes to mammalian cells
-
申请号: US14540816申请日: 2014-11-13
-
公开(公告)号: US09605031B2公开(公告)日: 2017-03-28
- 发明人: William M. Gelbart , Charles M. Knobler , Rees F. Garmann , Odisse Azizgolshani , Ruben D. Cadena-Nava
- 申请人: The Regents of the University of California
- 申请人地址: US CA Oakland
- 专利权人: The Regents of the University of California
- 当前专利权人: The Regents of the University of California
- 当前专利权人地址: US CA Oakland
- 代理机构: Gates & Cooper LLP
- 主分类号: C07K14/005
- IPC分类号: C07K14/005 ; C12N7/04 ; C12N5/00
摘要:
The invention provides compositions of matter comprising a cowpea chlorotic mottle virus capsid protein (CCMV CP) and a ribonucleic acid, as well as methods for using such compositions. In such compositions, the cowpea chlorotic mottle virus capsid protein envelops the ribonucleic acid so as to for a capsid that can inhibit the degradation of the ribonucleic acid (e.g. by RNAses). A method of delivering a ribonucleic acid into the cytoplasm of a mammalian cell is also provided. Typically, the method comprises the steps of combining the mammalian cell with a composition of matter described herein under conditions selected to allow the cowpea chlorotic mottle virus capsid to contact the mammalian cell and deliver the ribonucleic acid into the cytoplasm of a mammalian cell.
公开/授权文献
信息查询