Invention Grant
- Patent Title: Nicotinamide adenine dinucleotide gene encoding fluorescent probe, preparation method therefor and application thereof
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Application No.: US14347575Application Date: 2012-09-26
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Publication No.: US09606121B2Publication Date: 2017-03-28
- Inventor: Yi Yang , Yuzheng Zhao , Jing Jin , Qingxun Hu
- Applicant: East China University of Science and Technology
- Applicant Address: CN
- Assignee: East China University of Science and Technology
- Current Assignee: East China University of Science and Technology
- Current Assignee Address: CN
- Agency: Hovey Williams LLP
- Priority: CN201110288807 20110926
- International Application: PCT/CN2012/081977 WO 20120926
- International Announcement: WO2013/044792 WO 20130404
- Main IPC: G01N33/573
- IPC: G01N33/573 ; C07K14/195 ; C07K14/32 ; G01N33/50 ; G01N33/58
Abstract:
The invention relates to a genetically encoded fluorescent sensor for nicotinamide adenine dinucleotide, as well as methods of preparation and uses thereof. In one aspect, this invention relates to a sensor for detecting nicotinamide adenine dinucleotide, particularly, a recombinant fluorescent fusion protein sensor for detecting nicotinamide adenine dinucleotide. In one specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting reduced nicotinamide adenine dinucleotide (NADH); in another specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting oxidized nicotinamide adenine dinucleotide (NAD+); in yet another aspect, the invention relates to a recombinant fluorescent fusion protein sensor for detecting the ratio of reduced to oxidized nicotinamide adenine dinucleotide. This invention also relates to the method of preparing the sensors, and uses of the sensors in detecting NADH, NAD+, NADH/NAD+ ratio, screening drugs and measuring NADH metabolism.
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