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    Nicotinamide Adenine Dinucleotide Gene Encoding Fluorescent Probe, Preparation Method Therefor and Application Thereof
    3.
    发明申请
    Nicotinamide Adenine Dinucleotide Gene Encoding Fluorescent Probe, Preparation Method Therefor and Application Thereof 有权
    烟酰胺腺嘌呤二核苷酸基因编码荧光探针及其制备方法及应用

    公开(公告)号：US20140329718A1

    公开(公告)日：2014-11-06

    申请号：US14347575

    申请日：2012-09-26

    Applicant: East China University of Science and Technology

    Inventor： Yi Yang ,  Jing Jin ,  Yuzheng Zhao ,  Qingxun Hu

    IPC: G01N33/573 ,  G01N33/58 ,  G01N33/50

    CPC classification number: G01N33/5735 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00 ,  C07K2319/60 ,  G01N33/5011 ,  G01N33/582 ,  G01N2500/02 ,  G01N2800/7028

    Abstract: The invention relates to a genetically encoded fluorescent sensor for nicotinamide adenine dinucleotide, as well as methods of preparation and uses thereof. In one aspect, this invention relates to a sensor for detecting nicotinamide adenine dinucleotide, particularly, a recombinant fluorescent fusion protein sensor for detecting nicotinamide adenine dinucleotide. In one specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting reduced nicotinamide adenine dinucleotide (NADH); in another specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting oxidized nicotinamide adenine dinucleotide (NAD+); in yet another aspect, the invention relates to a recombinant fluorescent fusion protein sensor for detecting the ratio of reduced to oxidized nicotinamide adenine dinucleotide. This invention also relates to the method of preparing the sensors, and uses of the sensors in detecting NADH, NAD+, NADH/NAD+ ratio, screening drugs and measuring NADH metabolism.

    Abstract translation: 本发明涉及用于烟酰胺腺嘌呤二核苷酸的遗传编码荧光传感器，以及其制备方法和用途。 一方面，本发明涉及用于检测烟酰胺腺嘌呤二核苷酸的传感器，特别是用于检测烟酰胺腺嘌呤二核苷酸的重组荧光融合蛋白传感器。 在一个具体方面，本发明涉及用于检测还原型烟酰胺腺嘌呤二核苷酸（NADH）的重组荧光融合蛋白传感器。 在另一具体方面，本发明涉及用于检测氧化烟酰胺腺嘌呤二核苷酸（NAD +）的重组荧光融合蛋白传感器。 在另一方面，本发明涉及用于检测还原氧化烟酰胺腺嘌呤二核苷酸比例的重组荧光融合蛋白传感器。 本发明还涉及传感器的制备方法，以及传感器检测NADH，NAD +，NADH / NAD +比例，筛选药物和测定NADH代谢的用途。

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