An object of the present invention is to provide a tire puncture sealing agent that has excellent freeze resistance and that also exhibits excellent sealing properties even in a small liquid amount. The tire puncture sealing agent of the present invention contains: a rubber latex and/or a resin emulsion; a glycol ether represented by Formula (1) below; and a surfactant. In Formula (1), R11 represents a hydrogen atom or an alkyl group. R12 represents an alkyl group. p represents an integer of 1 or greater.