发明授权
- 专利标题: Baculoviral DNA elements for the expression of recombinant proteins in a host insect
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申请号: US14407709申请日: 2012-06-12
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公开(公告)号: US09701983B2公开(公告)日: 2017-07-11
- 发明人: Silvia Gomez Sebastian , Javier López Vidal , José Angel Martinez Escribano
- 申请人: Silvia Gomez Sebastian , Javier López Vidal , José Angel Martinez Escribano
- 申请人地址: ES Madrid
- 专利权人: ALTERNATIVE GENE EXPRESSION S.L.
- 当前专利权人: ALTERNATIVE GENE EXPRESSION S.L.
- 当前专利权人地址: ES Madrid
- 代理机构: McCarter & English, LLP
- 代理商 Danielle L. Herritt; Jill Ann Mello
- 国际申请: PCT/EP2012/061088 WO 20120612
- 国际公布: WO2012/168493 WO 20121213
- 主分类号: A01N63/00
- IPC分类号: A01N63/00 ; C12N15/00 ; C12Q1/68 ; C12N15/86 ; A01K67/033
摘要:
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters.
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