A method of positioning a mobile station (MS) in a wireless communication system is provided. The method is performed by the MS. The method includes receiving information on a reference signal for positioning from a serving base station, measuring the reference signal, comparing a transmission polarization characteristic and reception polarization characteristic of the measured reference signal and determining whether a light of sight (LOS) link between a base station for transmitting the reference signal and the MS is present or not, when the information on the reference signal includes information on the transmission polarization characteristic of the reference signal, and transmitting information on whether the LOS link is present or not to the serving base station.