- 专利标题: Production of anti-peptide antibodies
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申请号: US13990344申请日: 2011-11-29
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公开(公告)号: US09745353B2公开(公告)日: 2017-08-29
- 发明人: Joseph Krebs , Paul Morrison , Jun Wang
- 申请人: Joseph Krebs , Paul Morrison , Jun Wang
- 申请人地址: US TX Austin
- 专利权人: Bioo Scientific Corporation
- 当前专利权人: Bioo Scientific Corporation
- 当前专利权人地址: US TX Austin
- 代理机构: Meyertons, Hood, Kivlin, Kowert & Goetzel, P.C.
- 国际申请: PCT/US2011/062318 WO 20111129
- 国际公布: WO2012/074966 WO 20120607
- 主分类号: C07K14/195
- IPC分类号: C07K14/195 ; C07K16/00 ; A61K39/00 ; C07K19/00 ; C07K16/40 ; C12N9/02
摘要:
Anti-peptide antibodies (APAs) are extremely important tools for biomedical research. Many important techniques, such as immunoblots, ELISA immunoassays, immunocytochemistry, and protein microarrays are intrinsically linked to APA function and completely dependent on APA quality. Unfortunately, not all commercially-available APAs have good antigen binding characteristics; as a result, researchers are often unable to perform high quality protein analysis experiments. This disclosure describes a new method for the scalable production of polyclonal APAs using recombinant antigens. These recombinant peptide antigens have several advantages over traditional peptide antigens which improve the ease and speed of antibody production. The recombinant antigens can be scalably produced and purified much faster than traditional synthetic peptide-conjugates. These recombinant antigen-carriers are designed to specifically aggregate in vivo after administration into the host; this aggregation greatly enhances immunogenicity and may eliminate the need for the use of chemical adjuvants which cause physical irritation and discomfort to the host.
公开/授权文献
- US20140046035A1 IMPROVED PRODUCTION OF ANTI-PEPTIDE ANTIBODIES 公开/授权日:2014-02-13