- 专利标题： Seed-specific expression vector and its construction methods and applications
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申请号： US13515209申请日： 2010-12-09
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公开(公告)号： US09745362B2公开(公告)日： 2017-08-29
- 发明人: Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Liangyuan Hu , Haimin Xu , Chenggang Yu
- 申请人： Shengjun An , Xiqing Chai , Kunsheng Wang , Tiemei Shao , Zhan Jiao , Xin Wen , Xue Li , Pei Liu , Haigang Lu , Yunyu Chen , Liangyuan Hu , Haimin Xu , Chenggang Yu
- 申请人地址： CN Hebei CN Beijing CN Hebei
- 专利权人： Shengjun An,Kunsheng Wang,Xiqing Chai
- 当前专利权人： Shengjun An,Kunsheng Wang,Xiqing Chai
- 当前专利权人地址： CN Hebei CN Beijing CN Hebei
- 代理机构： Lackenbach Siegel, LLP
- 代理商 Andrew F. Young, Esq.
- 优先权： CN200910250703 20091209
- 国际申请： PCT/CN2010/079620 WO 20101209
- 国际公布： WO2011/069459 WO 20110616
- 主分类号： C07K14/775
- IPC分类号： C07K14/775 ; C12N15/82
摘要：
A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.
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