公开(公告)号：US09745362B2

公开(公告)日：2017-08-29

申请号：US13515209

申请日：2010-12-09

申请人： Shengjun An ,  Xiqing Chai ,  Kunsheng Wang ,  Tiemei Shao ,  Zhan Jiao ,  Xin Wen ,  Xue Li ,  Pei Liu ,  Haigang Lu ,  Yunyu Chen ,  Liangyuan Hu ,  Haimin Xu ,  Chenggang Yu

发明人： Shengjun An ,  Xiqing Chai ,  Kunsheng Wang ,  Tiemei Shao ,  Zhan Jiao ,  Xin Wen ,  Xue Li ,  Pei Liu ,  Haigang Lu ,  Yunyu Chen ,  Liangyuan Hu ,  Haimin Xu ,  Chenggang Yu

IPC分类号： C07K14/775 ,  C12N15/82

CPC分类号： C07K14/775 ,  C12N15/8257

摘要： A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.

公开(公告)号：US20120288893A1

公开(公告)日：2012-11-15

申请号：US13515209

申请日：2010-12-09

申请人： Shengjun An ,  Xiqing Chai ,  Kunsheng Wang ,  Tiemei Shao ,  Zhan Jiao ,  Xin Wen ,  Xue Li ,  Pei Liu ,  Haigang Lu ,  Yunyu Chen ,  Lingyuan Hu ,  Haimin Xu ,  Chenggang Yu

发明人： Shengjun An ,  Xiqing Chai ,  Kunsheng Wang ,  Tiemei Shao ,  Zhan Jiao ,  Xin Wen ,  Xue Li ,  Pei Liu ,  Haigang Lu ,  Yunyu Chen ,  Lingyuan Hu ,  Haimin Xu ,  Chenggang Yu

IPC分类号： C12N15/82 ,  C12P21/00 ,  C12P19/34

CPC分类号： C07K14/775 ,  C12N15/8257

摘要： A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.

摘要翻译： 公开了种子特异性表达载体及其构建方法和应用。 由植物二元表达载体pBI121的HindIII和SacI位点之间插入由油菜籽油质蛋白基因启动子驱动的花生梭菌油质蛋白基因 - 载脂蛋白A-IMilano（A-IM）基因组成的融合蛋白表达盒，获得植物表达载体 本发明的pBINOA。 此外，提供了一种生产载脂蛋白A-IMilano的方法，其中表达载体用于转化用作植物生物反应器的油向日葵。 该方法不仅可以提高载脂蛋白A-IMilano的产量，而且大大降低生产成本，适用于工业生产。