A video quality objective assessment method based on a spatiotemporal domain structure firstly combines a spatiotemporal domain gradient magnitude and color information for calculating a spatiotemporal domain local similarity, and then uses variance fusion for spatial domain fusion. The spatiotemporal domain local similarity is fused into frame-level objective quality value, and then a temporal domain fusion model is established by simulating three important global temporal effects, which are a smoothing effect, an asymmetric track effects and a recency effect, of a human visual system. Finally, the objective quality values of the distorted video sequence are obtained. By modeling the human visual temporal domain effect, the temporal domain weighting method of the present invention is able to accurately and efficiently evaluate the objective quality of the distorted video.