Invention Grant
- Patent Title: Method for refolding antibody, process for producing refolded antibody, refolded antibody, and uses thereof
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Application No.: US14767373Application Date: 2014-02-03
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Publication No.: US09850316B2Publication Date: 2017-12-26
- Inventor: Yoichi Kumada , Yasuyuki Ishikawa , Yusuke Fujiwara , Michimasa Kishimoto
- Applicant: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY
- Applicant Address: JP Kyoto
- Assignee: National University Corporation Kyoto Institute of Technology
- Current Assignee: National University Corporation Kyoto Institute of Technology
- Current Assignee Address: JP Kyoto
- Agency: MH2 Technology Law Group, LLP
- Priority: JP2013-027862 20130215
- International Application: PCT/JP2014/052475 WO 20140203
- International Announcement: WO2014/125955 WO 20140821
- Main IPC: C07K16/18
- IPC: C07K16/18 ; C07K16/26 ; C07K17/14 ; C07K17/08 ; C07K16/42 ; C07K16/06 ; C07K16/30 ; C07K16/40 ; C08F122/10
Abstract:
A method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof are provided. A method for refolding an antibody in a liquid phase comprises the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. Also provided is a process for producing a refolded antibody.
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