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    PEPTIDE HAVING AFFINITY FOR SILICON NITRIDE (SI3N4), AND USE THEREOF
    3.
    发明申请
    PEPTIDE HAVING AFFINITY FOR SILICON NITRIDE (SI3N4), AND USE THEREOF 有权
    具有氮化硅（SI3N4）的亲水性肽及其用途

    公开(公告)号：US20150038675A1

    公开(公告)日：2015-02-05

    申请号：US14378433

    申请日：2013-02-12

    Applicant: National University Corporation Kyoto Institute of Technology

    Inventor： Yoichi Kumada ,  Michimasa Kishimoto ,  Takeru Otsuka

    IPC: C07K17/14 ,  C07K7/08 ,  C07K7/06 ,  C07K14/245

    CPC classification number: C07K17/14 ,  C07K7/06 ,  C07K7/08 ,  C07K14/245 ,  C07K2319/00 ,  C07K2319/23 ,  C12N9/0006 ,  C12N11/14

    Abstract: The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.

    Abstract translation: 本发明的目的是提供：对氮化硅具有亲和力的肽; 编码该肽的多核苷酸; 用于表达对氮化硅具有亲和性的肽的表达载体; 用于表达包含对氮化硅和靶蛋白具有亲和力的肽的肽融合蛋白的表达载体; 通过将表达载体导入宿主细胞获得的转化体; 从转化体获得的肽融合蛋白; 已经结合了对氮化硅具有亲和性的肽的氮化硅衬底; 将靶蛋白固定在氮化硅衬底上的方法; 用于将靶蛋白固定到氮化硅衬底的组合物，所述组合物包含对氮化硅具有亲和性的肽; 以及用于将靶蛋白固定到氮化硅衬底的接头，所述接头包含对氮化硅具有亲和性的肽。 本发明涉及对氮化硅具有亲和力的肽，所述肽包含（1-1）具有SEQ ID NO：1,2和23至27之一的氨基酸序列的肽，（1-2）肽， 对氮化硅具有亲和性，并且包含通过在（1-1）所示的氨基酸序列之一中缺失，添加和/或取代一个或多个氨基酸而获得的氨基酸序列，或其中一个肽的一个片段 。

    Peptide having affinity for silicon nitride (Si3N4), and use therefor
    5.
    发明授权
    Peptide having affinity for silicon nitride (Si3N4), and use therefor 有权

    公开(公告)号：US09701760B2

    公开(公告)日：2017-07-11

    申请号：US14378433

    申请日：2013-02-12

    Applicant: National University Corporation Kyoto Institute of Technology

    Inventor： Yoichi Kumada ,  Michimasa Kishimoto ,  Takeru Otsuka

    IPC: C07K17/14 ,  C07K14/245 ,  C07K7/06 ,  C07K7/08 ,  C12N9/04 ,  C12N11/14

    CPC classification number: C07K17/14 ,  C07K7/06 ,  C07K7/08 ,  C07K14/245 ,  C07K2319/00 ,  C07K2319/23 ,  C12N9/0006 ,  C12N11/14

    Abstract: The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.

    ANTIBODY-IMMOBILIZED CARRIER, METHOD OF PRODUCING ANTIBODY-IMMOBILIZED CARRIER, AND USE OF SAID ANTIBODY-IMMOBILIZED CARRIER
    6.
    发明申请
    ANTIBODY-IMMOBILIZED CARRIER, METHOD OF PRODUCING ANTIBODY-IMMOBILIZED CARRIER, AND USE OF SAID ANTIBODY-IMMOBILIZED CARRIER 有权
    抗体固定载体，抗体载体的制备方法和抗体载体的使用

    公开(公告)号：US20150018534A1

    公开(公告)日：2015-01-15

    申请号：US14502013

    申请日：2014-09-30

    Applicant: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY ,  DENKA SEIKEN CO., LTD.

    Inventor： Yoichi Kumada ,  Michimasa Kishimoto ,  Kyoko Hamasaki ,  Aya Nakagawa

    IPC: C07K17/00

    CPC classification number: C07K17/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/249 ,  C07K16/2887 ,  C07K16/303 ,  C07K16/40 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55

    Abstract: The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.

    Abstract translation: 本发明提供可用于抗体筛选的抗体固定化载体，产生抗体固定化载体的方法和抗体固定化载体的用途。 特别是通过包含两个或更多个抗体固定化区域的抗体固定化载体，其中分别固定有重链低分子量抗体和轻链低分子量抗体，能够有效地进行抗体筛选， 两个或更多个抗体固定化区域各自以独立的方式包含，重链低分子量抗体包括重链可变区，轻链低分子量抗体包含轻链可变区 ，重链低分子量抗体和轻链低分子量抗体各自衍生自识别不同抗原的抗体。

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