An enhanced globulin-1 regulatory region is shown, a nucleotide sequence of which includes at least one additional copy of a region of the globulin-1 regulatory region which includes at least one transcriptional factor binding domain, combined with a transcription initiation site and translation start site. The promoter provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant.