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公开(公告)号：US20230002780A1
公开(公告)日：2023-01-05
申请号：US17808901
申请日：2022-06-24
发明人： John Howard , Erin Egelkrout
IPC分类号： C12N15/82 , A61K39/215
摘要： A plant produced vaccine for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is provided where the Spike protein of the virus is expressed in a plant by introducing into a plant a construct comprising a promoter preferentially directing expression to seed of said plant, a nucleic acid encoding the Spike protein and a nucleic acid targeting expression to the endoplasmic reticulum of the plant. The plant expresses the 51 polypeptide at levels of at least 10 mg/kg of seed of said plant. When orally administered to an animal, a protective response is observed including a serum antibody response.
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公开(公告)号：US20220195449A1
公开(公告)日：2022-06-23
申请号：US17455871
申请日：2021-11-19
摘要： Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.
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公开(公告)号：US11090380B2
公开(公告)日：2021-08-17
申请号：US16423839
申请日：2019-05-28
发明人： John Howard , Celine Hayden
摘要： Methods of vaccination of animals are provided. In an embodiment, a paired administration of a primer vaccine provides for non-oral administration of a vaccine and an oral administration of the vaccine and can be followed by a paired administration of a booster vaccine of a non-oral administration and an oral administration. Embodiments provide the non-oral and oral administration are within three days of each other. The methods provide for improved protective response in an animal compared to administration of a primer non-oral administered vaccine followed by three booster non-oral administered vaccines. An adjuvant is provided that is a microcrystalline polysaccharide-based adjuvant derived from delta inulin.
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公开(公告)号：US08115060B2
公开(公告)日：2012-02-14
申请号：US12390485
申请日：2009-02-22
申请人： John Howard , Erin Engelkrout
发明人： John Howard , Erin Engelkrout
CPC分类号： C12N15/8234 , C07K14/36 , C07K14/465 , C12N15/8267
摘要： A method of controlling germination of seed is provided in which a nucleic acid encoding a protein which causes inhibition of germination is transformed into a plant, operably linked with a promoter that expresses during germination. The protein is one in which a restoring substance can be applied to the seed and restores germination. Also provided is an amylase germination preferred promoter.
摘要翻译： 提供了一种控制种子发芽的方法，其中将编码引起抑制发芽的蛋白质的核酸转化到植物中，与在发芽过程中表达的启动子可操作地连接。 该蛋白质可以将恢复物质应用于种子并恢复萌发。 还提供了淀粉酶发芽优选的启动子。
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公开(公告)号：US09441232B2
公开(公告)日：2016-09-13
申请号：US13788731
申请日：2013-03-07
发明人： John Howard , Erin Engelkrout
IPC分类号： C12N15/82 , C07K14/425
CPC分类号： C12N15/8234 , C07K14/425 , C12N15/8241 , C12N15/8257
摘要： A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly pericarp preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the pericarp tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the pericarp of monocotyledonous plants, particularly cereals, and maize.
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6.发明申请METHODS TO INCREASE ANTIGENICITY OF MEMBRANE-BOUND POLYPEPTIDES PRODUCED IN PLANTS 审中-公开增加植物中生产的膜结合多肽的抗原性的方法公开(公告)号：US20140205625A1
公开(公告)日：2014-07-24
申请号：US14198712
申请日：2014-03-06
IPC分类号： C07K14/415
CPC分类号： C12N15/8257 , A61K39/12 , A61K39/21 , A61K39/292 , A61K2039/517 , A61K2039/542 , A61K2039/545 , A61K2039/55544 , C12N15/8258 , C12N2740/16034
摘要： Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.
摘要翻译： 在从植物中提取多肽或其它化合物使得磷脂与多肽相关联的过程中提供了从植物产生的膜结合多肽的增加的抗原性。 通过超临界流体萃取降低脂肪能增加植物产生的膜结合多肽的抗原性。 提供了产生这种膜结合多肽的方法和方法。 通过向动物施用膜结合的多肽来提供在动物中产生保护性应答的方法。 对膜结合多肽的特异性抗体的结合增加。 该方法提供了膜结合多肽的优选形成。 当植物材料脱脂时，膜结合多肽的稳定性增加。
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公开(公告)号：USRE41318E1
公开(公告)日：2010-05-04
申请号：US11809900
申请日：2007-06-01
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8216 , C12N15/8222
摘要： The invention discloses novel promoter sequences capable of expressing genes in plant cells. The promoters include engineered versions of the maize ubiquitin promoter to increase expression levels beyond those observed with the native ubiquitin promoter and alter the tissue preference. Expression constructs, vectors, transgenic plants and methods are also disclosed.
摘要翻译： 本发明公开了能够在植物细胞中表达基因的新型启动子序列。 启动子包括玉米泛素启动子的工程化版本，以增加超过用天然泛素启动子观察到的表达水平，并改变组织偏好。 还公开了表达构建体，载体，转基因植物和方法。
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公开(公告)号：US11124799B2
公开(公告)日：2021-09-21
申请号：US16439301
申请日：2019-06-12
发明人： John Howard , Celine Hayden
摘要： Vaccines, methods of producing, and methods of using are provided in which a protective response to Valley Fever disease is produced when administered to an animal. The vaccine provides for expression of Coccidioides sp. Ag2 polypeptide in a plant or plant part, linked to a promoter preferentially directing expression to seed tissue of the plant or plant part. Further embodiments provide the polypeptide is targeted to the cell wall, vacuole or endoplasmic reticulum. The polypeptide may be fused to a dendritic cell targeting dendritic cell or a heat labile enterotoxin. Increased expression levels in the plant or plant part are obtained. The vaccine comprising the plant-produced Ag2 polypeptide may be a glucan chitin particle comprising the Ag2 polypeptide. The plant or plant materials in an embodiment may be orally administered.
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公开(公告)号：US10150970B2
公开(公告)日：2018-12-11
申请号：US14198712
申请日：2014-03-06
摘要： Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.
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公开(公告)号：US08709761B2
公开(公告)日：2014-04-29
申请号：US13025659
申请日：2011-02-11
申请人： John Howard , Gina Fake
发明人： John Howard , Gina Fake
IPC分类号： C12P19/00
摘要： Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional β-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.
摘要翻译： 提供植物多糖的糖化，其中通过添加植物组织组合物获得从纤维素中释放可发酵糖。 获得葡萄糖的生产而不需要添加另外的葡糖苷酶。 使用纤维素降解组合物将植物组织组合物添加到纤维素降解组合物中以降解纤维素导致与不加植物组织组合物的方法相比，可发酵糖的产量增加。 在使用纤维素降解酶组合物降解纤维素的方法中使用植物组织组合物导致生产可发酵糖所需的纤维素降解酶组合物或外源施用的纤维素酶的量减少。
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