A lane within a processor bus that communicatively connects a transmitter and a receiver is dynamically assigned as a clock lane. The clock lane subsequently transmits a reference clock signal to coordinate data communications from the transmitter to the receiver. The clock lane may be assigned by determining signal margins of various lanes of the processor bus. The signal margins are determined by the transmitter sending a test pattern upon the various lanes and analyzing the received test pattern at the receiver. A dynamically assigned clock lane results increased overall signal integrity of communications between the transmitter and receiver. Further, a dynamically assigned clock lane may result in different lanes being assigned as the clock lane at distinct boot up instances, adding to the complexity of the unauthorized user determining the operational logic of the transmitter.