An organic light emitting diode display panel and a fabrication method thereof, a display device are provided, and the method includes: forming an anode layer (41) a light-emitting layer and a cathode layer on a substrate (40) and the method further includes: depositing a first pixel defining layer thin film (51) on the anode layer (41); depositing a second pixel defining layer thin film on the first pixel defining layer thin film (51), and forming a second pixel defining layer (61) by patterning the second pixel defining layer thin film through patterning process; and forming a first pixel defining layer (71) through dry etching and removing the first pixel defining layer thin film that is exposed; wherein the first pixel defining layer (71) has a lyophilic property and the second pixel defining layer (61) has a lyophobic property. A double-layer pixel defining layer prepared in the method is capable of reducing a mask plate, reducing the production cost, and improving the production efficiency.