Invention Application
WO2010117457A2 SYSTEM COMPRISING DUAL-SIDED THERMAL CYCLER AND EMULSION PCR IN POUCH
审中-公开
系统包含双面热循环和乳液中的乳液PCR
- Patent Title: SYSTEM COMPRISING DUAL-SIDED THERMAL CYCLER AND EMULSION PCR IN POUCH
- Patent Title (中): 系统包含双面热循环和乳液中的乳液PCR
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Application No.: PCT/US2010/001053Application Date: 2010-04-08
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Publication No.: WO2010117457A2Publication Date: 2010-10-14
- Inventor: LIU, David , YANG, Joon, Mo , SHARIATI, Maryam , WOO, Sam , COX, David, M. , LEHTO, Dennis
- Applicant: APPLIED BIOSYSTEMS, LLC , LIU, David , YANG, Joon, Mo , SHARIATI, Maryam , WOO, Sam , COX, David, M. , LEHTO, Dennis
- Applicant Address: 5781 Van Allen Way Carlsbad, CA 92008 US
- Assignee: APPLIED BIOSYSTEMS, LLC,LIU, David,YANG, Joon, Mo,SHARIATI, Maryam,WOO, Sam,COX, David, M.,LEHTO, Dennis
- Current Assignee: APPLIED BIOSYSTEMS, LLC,LIU, David,YANG, Joon, Mo,SHARIATI, Maryam,WOO, Sam,COX, David, M.,LEHTO, Dennis
- Current Assignee Address: 5781 Van Allen Way Carlsbad, CA 92008 US
- Agency: BOWERSOX, Leonard, D.
- Priority: US61/167,781 20090408; US61/167,766 20090408; US61/307,428 20100223
- Main IPC: C12M1/38
- IPC: C12M1/38 ; C12Q1/68
Abstract:
A system and method are provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions. A sample is retained in a pouch or flexible bag which permits bulk PCR amplification with efficient heat-transfer properties. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.
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