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公开(公告)号：WO2011026136A1
公开(公告)日：2011-03-03
申请号：PCT/US2010/047392
申请日：2010-08-31
Applicant: LIFE TECHNOLOGIES CORPORATION , HOSHIZAKI, Jon , YANG, Joon Mo , SHARIATI, Maryam , COX, David , HIRANO, Kirk , BRIDGHAM, John , WOO, Sam , GOLDA, George
Inventor： HOSHIZAKI, Jon , YANG, Joon Mo , SHARIATI, Maryam , COX, David , HIRANO, Kirk , BRIDGHAM, John , WOO, Sam , GOLDA, George
CPC classification number: C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 µl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.
Abstract translation: 本文提供了低体积测序系统的各种实施方案。 该系统可以包括具有至少一个具有确定体积（例如小于约100μl）的反应室的低体积流动池。 该系统还可以包括配置成与对应于目标反应室的入口端可逆耦合的自动化试剂输送机构，从而使试剂能够以最小的试剂废物从试剂容器准确地移动到反应室。 流通池可以包括多个反应室（例如6），从而允许多个样品的并行分析。 本文还提供了分析生物分子的各种方法。
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公开(公告)号：WO2009079052A3
公开(公告)日：2009-08-20
申请号：PCT/US2008077092
申请日：2008-09-19
Applicant: APPLIED BIOSYSTEMS , YANG JOON MO , LIU DAVID M , CHIANG YUH-MIN , SCHEMBRI CAROL , LAU ALDRICH N K , ULMANELLA UMBERTO , BEARD NIGEL P , SHARIATI MARYAM , NURSE JAMES C , HAGA DOUWE D , HARDING IAN A , FOCARACCI JULIO P
Inventor： YANG JOON MO , LIU DAVID M , CHIANG YUH-MIN , SCHEMBRI CAROL , LAU ALDRICH N K , ULMANELLA UMBERTO , BEARD NIGEL P , SHARIATI MARYAM , NURSE JAMES C , HAGA DOUWE D , HARDING IAN A , FOCARACCI JULIO P
IPC: B01L3/00
CPC classification number: B01L3/502707 , B01L7/52 , B01L2200/141 , B01L2300/0816 , B01L2300/1805 , B01L2300/1861 , B01L2400/0677
Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.
Abstract translation: 描述了用于在测试卡上隔离室的测定卡和装置和方法。 测定卡包括由一种或多种具有软化温度的材料（例如塑料）形成的衬底，衬底限定通道与各个反应室连通。 测定卡可以在通道的区域中被加热到至少软化温度。 软化的塑料可能变形，例如用可能也可能不提供用于软化基底的热量的工具。 以这种方式，可以使衬底的塑料至少部分地阻碍通道，从而隔离反应室。 本发明还涉及一种制造工具装置的方法，该工具装置包括用于加热和变形分析卡的销。
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3.发明申请FAST - INDEXING FILTER WHEEL AND ITS USE IN A NUCLEIC ACID SEQUENCING SYSTEM 审中-公开快速 - 引导过滤轮及其在核酸序列测序系统中的应用
公开(公告)号：WO2011026141A3
公开(公告)日：2011-04-21
申请号：PCT/US2010047402
申请日：2010-08-31
Applicant: LIFE TECHNOLOGIES CORP , COX DAVID , HABERSTROH MARC , KINNEY PATRICK D , CARRILLO ALBERT L , HOSHIZAKI JON A , SHARIATI MARYAM , KING HOWARD G , LEE JOE Y , CHAN MATTHEW S
Inventor： COX DAVID , HABERSTROH MARC , KINNEY PATRICK D , CARRILLO ALBERT L , HOSHIZAKI JON A , SHARIATI MARYAM , KING HOWARD G , LEE JOE Y , CHAN MATTHEW S
CPC classification number: C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast- indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser). For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast- indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelength are also provided herein.
Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括快速分度滤光轮，其具有能够快速旋转进入激发源（例如，弧光灯，激光器）和与激光源（例如，弧光灯，激光器）连通的多个激发和发射滤光片。 例如，滤光轮可被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。 该系统还可以配置为考虑快速启动和停止快速分度滤光轮以及其他来源产生振动的振动。 本文还提供了用于快速成像多个波长的样品的各种方法。
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公开(公告)号：WO2011026141A2
公开(公告)日：2011-03-03
申请号：PCT/US2010/047402
申请日：2010-08-31
Applicant: LIFE TECHNOLOGIES CORPORATION , COX, David , HABERSTROH, Marc , KINNEY, Patrick D. , CARRILLO, Albert L. , HOSHIZAKI, Jon A. , SHARIATI, Maryam , KING, Howard G. , LEE, Joe Y. , CHAN, Matthew S.
Inventor： COX, David , HABERSTROH, Marc , KINNEY, Patrick D. , CARRILLO, Albert L. , HOSHIZAKI, Jon A. , SHARIATI, Maryam , KING, Howard G. , LEE, Joe Y. , CHAN, Matthew S.
CPC classification number: C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source ( e.g. , an arc lamp, a laser). For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.
Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括具有多个激发和发射滤波器的快速分度滤光轮，该激发和发射滤光片能够快速旋转进入激发源（例如，弧光灯，激光器）和与激发源（例如，弧光灯，激光器）连通。 例如，滤光轮可被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。 该系统还可以被配置为考虑快速启动和停止快速分度滤光轮以及由其他来源产生的振动所产生的振动。 本文还提供了在多个波长下快速成像样品的各种方法。
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5.发明申请
公开(公告)号：WO2010117457A2
公开(公告)日：2010-10-14
申请号：PCT/US2010/001053
申请日：2010-04-08
Applicant: APPLIED BIOSYSTEMS, LLC , LIU, David , YANG, Joon, Mo , SHARIATI, Maryam , WOO, Sam , COX, David, M. , LEHTO, Dennis
Inventor： LIU, David , YANG, Joon, Mo , SHARIATI, Maryam , WOO, Sam , COX, David, M. , LEHTO, Dennis
CPC classification number: B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481
Abstract: A system and method are provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions. A sample is retained in a pouch or flexible bag which permits bulk PCR amplification with efficient heat-transfer properties. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.
Abstract translation: 提供适用于常规基于PCR的反应以及基于乳液的PCR反应的大体积样品扩增的系统和方法。 将样品保留在袋或柔性袋中，其允许具有有效的热传递性质的大量PCR扩增。 对于涉及基于乳液的PCR扩增的应用，该系统和方法提供了乳液扩增的改进的均匀性，并可用于快速和可重复地扩增大体积或小体积的乳液。
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Patent Agency Ranking6.发明申请
公开(公告)号：WO2010117457A3
公开(公告)日：2011-03-24
申请号：PCT/US2010001053
申请日：2010-04-08
Applicant: APPLIED BIOSYSTEMS LLC , LIU DAVID , YANG JOON MO , SHARIATI MARYAM , WOO SAM , COX DAVID M , LEHTO DENNIS
Inventor： LIU DAVID , YANG JOON MO , SHARIATI MARYAM , WOO SAM , COX DAVID M , LEHTO DENNIS
CPC classification number: B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481
Abstract: A system and method are provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions. A sample is retained in a pouch or flexible bag which permits bulk PCR amplification with efficient heat-transfer properties. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.
Abstract translation: 提供适用于常规基于PCR的反应以及基于乳液的PCR反应的大体积样品扩增的系统和方法。 将样品保留在袋或柔性袋中，其允许具有有效的热传递性质的大量PCR扩增。 对于涉及基于乳液的PCR扩增的应用，该系统和方法提供了乳液扩增的改进的均匀性，并可用于快速和可重复地扩增大体积或小体积的乳液。
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公开(公告)号：WO2011026128A3
公开(公告)日：2011-03-03
申请号：PCT/US2010/047377
申请日：2010-08-31
Applicant: LIFE TECHNOLOGIES CORPORATION , HIRANO, Kirk , ANDERSEN, Mark , GONG, Jian , WOO, Sam , COX, David , YANG, Joon Mo , YUE, Min , SHARIATI, Maryam , BRIDGHAM, John , LIU, David
Inventor： HIRANO, Kirk , ANDERSEN, Mark , GONG, Jian , WOO, Sam , COX, David , YANG, Joon Mo , YUE, Min , SHARIATI, Maryam , BRIDGHAM, John , LIU, David
Abstract: Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.
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公开(公告)号：WO2011026128A2
公开(公告)日：2011-03-03
申请号：PCT/US2010047377
申请日：2010-08-31
Applicant: LIFE TECHNOLOGIES CORP , HIRANO KIRK , ANDERSEN MARK , GONG JIAN , WOO SAM , COX DAVID , YANG JOON MO , YUE MIN , SHARIATI MARYAM , BRIDGHAM JOHN , LIU DAVID
Inventor： HIRANO KIRK , ANDERSEN MARK , GONG JIAN , WOO SAM , COX DAVID , YANG JOON MO , YUE MIN , SHARIATI MARYAM , BRIDGHAM JOHN , LIU DAVID
CPC classification number: C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
Abstract: Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.
Abstract translation: 提供了各种流通池配置和系统以及制造和使用它们的方法。 流通池，系统和使用方法可用于进行测序反应和下一代测序方法。
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公开(公告)号：WO2006116616A2
公开(公告)日：2006-11-02
申请号：PCT/US2006/016060
申请日：2006-04-26
Applicant: APPLERA CORPORATION , YUE, Min , LIU, David M. , CHIANG, Yuh-Min , YANG, Joon Mo , LEHTO, Dennis , VANN, Charles S. , BEARD, Nigel P. , HARDING, Ian A. , VAN CAMP, John , DROMARETSKY, Alexander , ERMAKOV, Sergey V. , OLDHAM, Mark F. , ROY, Joy , SHARIATI, Maryam , ULMANELLA, Umberto
Inventor： YUE, Min , LIU, David M. , CHIANG, Yuh-Min , YANG, Joon Mo , LEHTO, Dennis , VANN, Charles S. , BEARD, Nigel P. , HARDING, Ian A. , VAN CAMP, John , DROMARETSKY, Alexander , ERMAKOV, Sergey V. , OLDHAM, Mark F. , ROY, Joy , SHARIATI, Maryam , ULMANELLA, Umberto
IPC: G01N33/00
CPC classification number: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
Abstract translation: 用于多个分析物检测的系统和方法包括用于分配包括衬底的生物样品的系统，其中所述衬底包括多个样品室，用于每个样品室的样品引入通道和用于每个样品室的排气通道。 该系统可以进一步包括包含在每个样品室中并被配置用于进入基底的生物样品的核酸分析的预加载试剂和被配置为与基底接触以密封每个样品室以便基本上防止 每个样品室中包含的样品从每个样品室流出。 底物可以由检测兼容和测定相容的材料构成。
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10.发明申请
公开(公告)号：WO2009079052A2
公开(公告)日：2009-06-25
申请号：PCT/US2008/077092
申请日：2008-09-19
Applicant: APPLIED BIOSYSTEMS INC. , YANG, Joon Mo , LIU, David, M. , CHIANG, Yuh-Min , SCHEMBRI, Carol , LAU, Aldrich, N. K. , ULMANELLA, Umberto , BEARD, Nigel, P. , SHARIATI, Maryam , NURSE, James, C. , HAGA, Douwe, D. , HARDING, Ian, A. , FOCARACCI, Julio, P.
Inventor： YANG, Joon Mo , LIU, David, M. , CHIANG, Yuh-Min , SCHEMBRI, Carol , LAU, Aldrich, N. K. , ULMANELLA, Umberto , BEARD, Nigel, P. , SHARIATI, Maryam , NURSE, James, C. , HAGA, Douwe, D. , HARDING, Ian, A. , FOCARACCI, Julio, P.
CPC classification number: B01L3/502707 , B01L7/52 , B01L2200/141 , B01L2300/0816 , B01L2300/1805 , B01L2300/1861 , B01L2400/0677
Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.
Abstract translation: 描述了用于隔离化验卡上的室的化验卡和装置以及方法。 测定卡包括由具有软化温度的一种或多种材料（例如塑料）形成的基底，基底限定与相应反应室连通的通道。 可以在通道的区域中将测定卡加热到至少软化温度。 软化的塑料可以变形，例如用可以或不可以提供软化基材的热量的工具。 以这种方式，可以使衬底的塑料至少部分地阻塞通道，从而隔离反应室。 本发明还涉及一种制造工具装置的方法，所述工具装置包括用于加热和使检定卡变形的销。
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