Durable calibration standards are described herein for inspection systems for manufactured vials, such as glass pharmaceutical vials, and methods of using the same. A grayscale calibration standard is provided for calibration of camera settings of imaging components in an inspection system. The grayscale calibration standard comprises a vial having a laser etched gradient image on a portion of the vial. A region of interest (ROI) calibration standard is provided for calibration of spatial difference of imaging components and to align imaging components in an inspection system to capture desired regions of interest. The ROI calibration standard comprises a vial comprising laser etchings on one or more portions of the vial, wherein the laser etchings comprise laser markings formed in a geometric pattern. By providing laser-marked calibration standards, the calibration standards may be used in many different modes of metrology.