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55 条记录 (耗时 0.032 秒)

    UNIDIRECTIONAL SEQUENCING USING PANDOMERS
    4.
    发明公开
    UNIDIRECTIONAL SEQUENCING USING PANDOMERS 审中-公开

    公开(公告)号：EP3733874A1

    公开(公告)日：2020-11-04

    申请号：EP20164391.3

    申请日：2013-11-07

    申请人： Genia Technologies, Inc.

    发明人： DAVIS, Randall ,  CHEN, Roger ,  BIBILLO, Arkadiusz ,  KORENBLUM, Daniel

    IPC分类号： C12Q1/6869 ,  G01N33/487

    摘要： The present invention relates to a method for nucleic acid sequencing, the method comprising: (a) providing a single stranded nucleic acid; (b) providing a plurality of probes; (c) polymerizing the plurality of probes in an order determined by hybridization of the hybridization moieties with the single stranded nucleic acid to be sequenced; (d) cleaving the cleavable groups to provide an expanded thread to be sequenced; (e) threading the expanded thread through a nanopore, wherein the gates prevent the expanded thread from threading through the nanopore in the reverse direction; and (f) detecting, with the aid of the nanopore, the loop structures of the expanded thread in the order determined by hybridization of the hybridization moieties with the single stranded nucleic acid to be sequenced, thereby sequencing the single stranded nucleic acid to be sequenced.

    ALPHA-HEMOLYSIN VARIANTS WITH ALTERED CHARACTERISTICS
    6.
    发明公开
    ALPHA-HEMOLYSIN VARIANTS WITH ALTERED CHARACTERISTICS 审中-公开
    ALPHA-溶血素变异体具有改变的特征

    公开(公告)号：EP3212810A2

    公开(公告)日：2017-09-06

    申请号：EP15855632.4

    申请日：2015-10-28

    申请人： Genia Technologies, Inc.

    发明人： DORWART, Michael ,  KORENBLUM, Daniel

    IPC分类号： C12Q1/68

    摘要： Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

    摘要翻译： 本文描述了具有选自T12R，T12K，N17R，N17K或T12和N17突变组合的至少一个突变的α-溶血素的变体。 一些实施方案中的变体可以进一步包含H144A。 α-溶血素变体具有减少的螺纹时间。

    NANOPORE-BASED SEQUENCING CHIPS USING STACKED WAFER TECHNOLOGY
    7.
    发明公开
    NANOPORE-BASED SEQUENCING CHIPS USING STACKED WAFER TECHNOLOGY 审中-公开
    NANOPORENBASIERTE CHIPSEQUENZIERUNG MIT GESTAPELTER WAFERTECHNOLOGIE

    公开(公告)号：EP3122891A1

    公开(公告)日：2017-02-01

    申请号：EP15769702.0

    申请日：2015-03-11

    申请人： Genia Technologies, Inc.

    发明人： TIAN, Hui

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12M1/34

    CPC分类号： G01N33/48721

    摘要： A nanopore based sequencing chip is disclosed. The sequencing chip comprises a first portion made from a first wafer. The first portion includes an array of nanopore cells. The first portion further includes a measurement circuit connected to one or more nanopore cells, the measurement circuit producing an output measurement signal. The first portion further includes one or more vias transmitting the output measurement signal. The sequencing chip further includes a second portion made from a second wafer, the second portion comprising one or more corresponding vias receiving the output measurement signal.

    摘要翻译： 公开了一种基于纳米孔的测序芯片。 测序芯片包括由第一晶片制成的第一部分。 第一部分包括纳米孔细胞阵列。 第一部分还包括连接到一个或多个纳米孔单元的测量电路，测量电路产生输出测量信号。 第一部分还包括传输输出测量信号的一个或多个通孔。 排序芯片还包括由第二晶片制成的第二部分，第二部分包括接收输出测量信号的一个或多个相应的通孔。

    NON-FARADAIC, CAPACITIVELY COUPLED MEASUREMENT IN A NANOPORE CELL ARRAY
    8.
    发明公开
    NON-FARADAIC, CAPACITIVELY COUPLED MEASUREMENT IN A NANOPORE CELL ARRAY 审中-公开
    西班牙尼泊尔 - 泽尔马蒂克的KAPAZITIV GEKOPPELTE NICHT-FARADAY-MESSUNG

    公开(公告)号：EP3058102A2

    公开(公告)日：2016-08-24

    申请号：EP14853691.5

    申请日：2014-09-11

    申请人： Genia Technologies, Inc.

    发明人： CHEN, Roger J.A.

    IPC分类号： C12Q1/68 ,  C40B20/00 ,  C40B20/04

    CPC分类号： G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

    摘要： A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

    摘要翻译： 公开了鉴定分子的方法。 通过在第一时间段期间将一个第一电压信号施加到一对电极而将一个分子吸引到纳米孔，其中第一电压信号引起通过纳米孔的第一离子电流，其指示分子的一部分的性质接近于 纳米孔。 在第二时段期间，通过在所述一对电极施加第二电压信号，从所述纳米孔释放所述分子，其中所述第二电压信号引起通过所述纳米孔的第二离子电流。 至少部分地基于通过包含第一离子电流和第二离子电流的纳米孔的净离子电流来确定第一周期和第二周期。

    NANOPORE ARRAYS
    9.
    发明公开
    NANOPORE ARRAYS 审中-公开
    NANOPORENARRAYS

    公开(公告)号：EP2954316A1

    公开(公告)日：2015-12-16

    申请号：EP14748849.8

    申请日：2014-01-28

    申请人： Genia Technologies, Inc.

    发明人： CHEN, Roger J.A. ,  FULLAGAR, David J.

    IPC分类号： G01N27/447

    CPC分类号： G01N33/48721

    摘要： A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

    摘要翻译： 公开了一种利用纳米孔阵列分析分子的方法，所述纳米孔阵列包括在芯片上的多个单元。 导致在多个电池的至少一部分中形成纳米孔。 评估纳米孔的第一物理测量。 确定是否使分子与纳米孔相互作用。 导致至少一部分纳米孔与分子相互作用。 评估表示分子性质的纳米孔的第二物理测量。 确定是否使纳米孔被重整，使得细胞可以重复使用以与另外的分子相互作用。