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    UNIVERSAL CAPTURE ARRAY FOR MULTIPLEXED SUBTYPE-SPECIFIC QUANTIFICATION AND STABILITY DETERMINATION OF INFLUENZA PROTEINS
    1.
    发明公开
    UNIVERSAL CAPTURE ARRAY FOR MULTIPLEXED SUBTYPE-SPECIFIC QUANTIFICATION AND STABILITY DETERMINATION OF INFLUENZA PROTEINS 审中-公开
    UNIVERSELLES ERFASSUNGSARRAY ZUR MULTIPLEX-SUBTYPSPEZIFISCHEN QUANTIFIZIERUNG UNDSTABILITÄTSBESTIMMUNGVON INFLUENZAPROTEINEN

    公开(公告)号：EP3152347A1

    公开(公告)日：2017-04-12

    申请号：EP14893964.8

    申请日：2014-06-04

    申请人： Indevr, Inc.

    发明人： ROWLEN, Kathy ,  KUCK, Laura, R.

    IPC分类号： C40B30/04

    CPC分类号： G01N33/56983 ,  G01N2333/11

    摘要： A universal array for multiplexed quantification of variable hemagglutinin such as subcomponents of multivalent annual influenza vaccines that is robust to variations in proteins such as mutations and is capable of quantifying degradation of proteins. Universal capture array (100) comprises one or more substrates (102) and a low-density microarray (104) of sub-arrays (108) comprising spots (106a-c). The microarray (104) is contacted with one or more targets (202) at one or more unknown concentrations, and bound complexes (203) are formed and subsequently quantified with a suitable method. Quantified signals are compared to calibration curves to obtain one or more un-known concentrations and/or quantify degradation of the one or more targets (202). Other embodiments are described and shown.

    摘要翻译： 用于多变量血液凝集素的多重定量的通用阵列，例如多价年流感疫苗的亚组分，其对诸如突变之类的蛋白质的变化是稳定的，并且能够定量蛋白质的降解。 通用捕获阵列（100）包括一个或多个衬底（102）和包括点（106a-c）的子阵列（108）的低密度微阵列（104）。 将微阵列（104）以一种或多种未知浓度与一种或多种靶（202）接触，形成结合复合物（203），并随后用合适的方法进行定量。 将量化的信号与校准曲线进行比较以获得一个或多个未知浓度和/或量化一个或多个靶（202）的降解。 描述和示出了其他实施例。

    LOW DENSITY MICROARRAYS FOR VACCINE RELATED PROTEIN QUANTIFICATION, POTENCY DETERMINATION AND EFFICACY EVALUATION
    3.
    发明公开
    LOW DENSITY MICROARRAYS FOR VACCINE RELATED PROTEIN QUANTIFICATION, POTENCY DETERMINATION AND EFFICACY EVALUATION 审中-公开
    对于疫苗蛋白定量，效果判断及效率等级低密度微阵列

    公开(公告)号：EP2718715A4

    公开(公告)日：2015-02-18

    申请号：EP12801262

    申请日：2012-06-12

    申请人： INDEVR INC

    发明人： ROWLEN KATHY L

    IPC分类号： G01N33/53 ,  G01N33/52 ,  G01N33/543 ,  G01N33/68

    CPC分类号： G01N33/56983 ,  G01N33/6845 ,  G01N2333/11

    摘要： Methods for the quantification of influenza HA proteins and anti-influenza antibodies for the fields of vaccine-related protein quantification, potency determination, and efficacy evaluation are provided. According to the technology, quantification is achieved by providing capture agents attached to an array in a series of decreasing concentrations. Serial dilutions of a reference material also may be introduced. The reference material within each solution binds to the capture agents on the array and is labeled with a label agent capable of producing a detectable signal used to construct a calibration curve. A target material of unknown concentration is introduced to a separate identical array, and the target material binds to the capture agents and also is labeled by a label agent to produce a detectable signal. The calibration curve based on the reference material is then utilized to determine the concentration of the target material without the need to perform replicate experiments.

    摘要翻译： 提供了流感HA蛋白和抗流感抗体疫苗相关蛋白定量，效价测定，和疗效评价领域的定量方法。 。根据该技术，定量是通过提供附着到阵列捕获剂，在一系列递减浓度的实现。 参考物质的系列稀释液可因此被引入。 每个溶液中的参考材料结合到阵列上的捕获剂和标记有能够产生用于构建的校准曲线的可检测信号的标记剂。 未知浓度的靶材料被引入到单独的相同阵列，并且所述目标材料结合到所述捕获剂，并且因此被标记试剂标记，以产生可检测的信号。 然后，基于参考材料的校准曲线被用来确定矿靶材料的浓度，而不进行重复实验的需要。

    UNIVERSAL CAPTURE ARRAY FOR MULTIPLEXED SUBTYPE-SPECIFIC QUANTIFICATION AND STABILITY DETERMINATION OF INFLUENZA PROTEINS
    5.
    发明公开
    UNIVERSAL CAPTURE ARRAY FOR MULTIPLEXED SUBTYPE-SPECIFIC QUANTIFICATION AND STABILITY DETERMINATION OF INFLUENZA PROTEINS 审中-公开

    公开(公告)号：EP3916390A1

    公开(公告)日：2021-12-01

    申请号：EP21169741.2

    申请日：2014-06-04

    申请人： Indevr, Inc.

    发明人： ROWLEN, Kathy ,  KUCK, Laura R.

    IPC分类号： G01N33/569

    摘要： A universal array for multiplexed quantification of variable hemagglutinin such as subcomponents of multivalent annual influenza vaccines that is robust to variations in proteins such as mutations and is capable of quantifying degradation of proteins. Universal capture array ( 100 ) comprises one or more substrates ( 102 ) and a low-density microarray ( 104 ) of sub-arrays ( 108 ) comprising spots ( 106a-c ). The microarray ( 104 ) is contacted with one or more targets ( 202 ) at one or more unknown concentrations, and bound complexes ( 203 ) are formed and subsequently quantified with a suitable method. Quantified signals are compared to calibration curves to obtain one or more unknown concentrations and/or quantify degradation of the one or more targets ( 202 ). Other embodiments are described and shown.

    LOW DENSITY MICROARRAYS FOR VACCINE RELATED PROTEIN QUANTIFICATION, POTENCY DETERMINATION AND EFFICACY EVALUATION
    6.
    发明授权
    LOW DENSITY MICROARRAYS FOR VACCINE RELATED PROTEIN QUANTIFICATION, POTENCY DETERMINATION AND EFFICACY EVALUATION 有权

    公开(公告)号：EP2718715B1

    公开(公告)日：2018-12-26

    申请号：EP12801262.2

    申请日：2012-06-12

    申请人： InDevR, Inc.

    发明人： ROWLEN, Kathy, L.

    IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/543 ,  G01N33/52

    CPC分类号： G01N33/56983 ,  G01N33/6845 ,  G01N2333/11

    摘要： Methods for the quantification of influenza HA proteins and anti-influenza antibodies for the fields of vaccine-related protein quantification, potency determination, and efficacy evaluation are provided. According to the technology, quantification is achieved by providing capture agents attached to an array in a series of decreasing concentrations. Serial dilutions of a reference material also may be introduced. The reference material within each solution binds to the capture agents on the array and is labeled with a label agent capable of producing a detectable signal used to construct a calibration curve. A target material of unknown concentration is introduced to a separate identical array, and the target material binds to the capture agents and also is labeled by a label agent to produce a detectable signal. The calibration curve based on the reference material is then utilized to determine the concentration of the target material without the need to perform replicate experiments.

    LOW DENSITY MICROARRAYS FOR VACCINE RELATED PROTEIN QUANTIFICATION, POTENCY DETERMINATION AND EFFICACY EVALUATION
    7.
    发明公开
    LOW DENSITY MICROARRAYS FOR VACCINE RELATED PROTEIN QUANTIFICATION, POTENCY DETERMINATION AND EFFICACY EVALUATION 审中-公开
    对于疫苗蛋白定量，效果判断及效率等级低密度微阵列

    公开(公告)号：EP2718715A2

    公开(公告)日：2014-04-16

    申请号：EP12801262.2

    申请日：2012-06-12

    申请人： Indevr, Inc.

    发明人： ROWLEN, Kathy, L.

    IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/543 ,  G01N33/52

    CPC分类号： G01N33/56983 ,  G01N33/6845 ,  G01N2333/11

    摘要： Methods for the quantification of influenza HA proteins and anti-influenza antibodies for the fields of vaccine-related protein quantification, potency determination, and efficacy evaluation are provided. According to the technology, quantification is achieved by providing capture agents attached to an array in a series of decreasing concentrations. Serial dilutions of a reference material also may be introduced. The reference material within each solution binds to the capture agents on the array and is labeled with a label agent capable of producing a detectable signal used to construct a calibration curve. A target material of unknown concentration is introduced to a separate identical array, and the target material binds to the capture agents and also is labeled by a label agent to produce a detectable signal. The calibration curve based on the reference material is then utilized to determine the concentration of the target material without the need to perform replicate experiments.

    NANOPARTICLE-MEDIATED SIGNAL AMPLIFICATION
    8.
    发明公开
    NANOPARTICLE-MEDIATED SIGNAL AMPLIFICATION 审中-公开
    纳米颗粒介导的信号增益

    公开(公告)号：EP2271769A2

    公开(公告)日：2011-01-12

    申请号：EP09726214.1

    申请日：2009-03-18

    申请人： Indevr, Inc.

    发明人： ROWLEN, Kathy, L. ,  TAYLOR, Amber, W.

    IPC分类号： C12Q1/68 ,  B82B3/00

    CPC分类号： C12Q1/6816 ,  C12Q1/6825 ,  C12Q2565/518 ,  C12Q2563/155 ,  C12Q2527/125

    摘要： There is described a new class or type of initiators for polymerization as a means of signal enhancement, nanoparticle initiators, and methods for amplifying signal resulting from recognition events, thereby enhancing the detection of those recognition events. Methods include amplification achieved through polymerization using a nanoparticle initiator conjugated recognition element that is not consumed during the reaction. The polymer formed as a result of the absorption of light by the nanoparticle initiator and introduction of reactive species into a surrounding polymerizable monomer solution occurs in a spatially-limited region directly surrounding the nanoparticle initiator and is indicative of the recognition event(s). In one embodiment, a semiconductor quantum dot nanoparticle initiator is utilized. In another embodiment, a metal nanoparticle is utilized. In another embodiment, the signal is detected without instrumentation. In yet another embodiment, the signal is detected via a transmission-based instrument which captures an image of the formed polymer.

    UNIVERSAL CAPTURE ARRAY FOR MULTIPLEXED SUBTYPE-SPECIFIC QUANTIFICATION AND STABILITY DETERMINATION OF INFLUENZA PROTEINS
    10.
    发明公开
    UNIVERSAL CAPTURE ARRAY FOR MULTIPLEXED SUBTYPE-SPECIFIC QUANTIFICATION AND STABILITY DETERMINATION OF INFLUENZA PROTEINS 审中-公开

    公开(公告)号：EP3152347A4

    公开(公告)日：2018-04-11

    申请号：EP14893964

    申请日：2014-06-04

    申请人： INDEVR INC

    发明人： ROWLEN KATHY ,  KUCK LAURA R

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56983 ,  G01N2333/11

    摘要： A universal array for multiplexed quantification of variable hemagglutinin such as subcomponents of multivalent annual influenza vaccines that is robust to variations in proteins such as mutations and is capable of quantifying degradation of proteins. Universal capture array (100) comprises one or more substrates (102) and a low-density microarray (104) of sub-arrays (108) comprising spots (106a-c). The microarray (104) is contacted with one or more targets (202) at one or more unknown concentrations, and bound complexes (203) are formed and subsequently quantified with a suitable method. Quantified signals are compared to calibration curves to obtain one or more un-known concentrations and/or quantify degradation of the one or more targets (202). Other embodiments are described and shown.