公开(公告)号：EP2718715B1

公开(公告)日：2018-12-26

申请号：EP12801262.2

申请日：2012-06-12

申请人： InDevR, Inc.

发明人： ROWLEN, Kathy, L.

IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/543 ,  G01N33/52

CPC分类号： G01N33/56983 ,  G01N33/6845 ,  G01N2333/11

摘要： Methods for the quantification of influenza HA proteins and anti-influenza antibodies for the fields of vaccine-related protein quantification, potency determination, and efficacy evaluation are provided. According to the technology, quantification is achieved by providing capture agents attached to an array in a series of decreasing concentrations. Serial dilutions of a reference material also may be introduced. The reference material within each solution binds to the capture agents on the array and is labeled with a label agent capable of producing a detectable signal used to construct a calibration curve. A target material of unknown concentration is introduced to a separate identical array, and the target material binds to the capture agents and also is labeled by a label agent to produce a detectable signal. The calibration curve based on the reference material is then utilized to determine the concentration of the target material without the need to perform replicate experiments.

公开(公告)号：EP2718715A2

公开(公告)日：2014-04-16

申请号：EP12801262.2

申请日：2012-06-12

申请人： Indevr, Inc.

发明人： ROWLEN, Kathy, L.

IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/543 ,  G01N33/52

CPC分类号： G01N33/56983 ,  G01N33/6845 ,  G01N2333/11

摘要： Methods for the quantification of influenza HA proteins and anti-influenza antibodies for the fields of vaccine-related protein quantification, potency determination, and efficacy evaluation are provided. According to the technology, quantification is achieved by providing capture agents attached to an array in a series of decreasing concentrations. Serial dilutions of a reference material also may be introduced. The reference material within each solution binds to the capture agents on the array and is labeled with a label agent capable of producing a detectable signal used to construct a calibration curve. A target material of unknown concentration is introduced to a separate identical array, and the target material binds to the capture agents and also is labeled by a label agent to produce a detectable signal. The calibration curve based on the reference material is then utilized to determine the concentration of the target material without the need to perform replicate experiments.

公开(公告)号：EP2271769A2

公开(公告)日：2011-01-12

申请号：EP09726214.1

申请日：2009-03-18

申请人： Indevr, Inc.

发明人： ROWLEN, Kathy, L. ,  TAYLOR, Amber, W.

IPC分类号： C12Q1/68 ,  B82B3/00

CPC分类号： C12Q1/6816 ,  C12Q1/6825 ,  C12Q2565/518 ,  C12Q2563/155 ,  C12Q2527/125

摘要： There is described a new class or type of initiators for polymerization as a means of signal enhancement, nanoparticle initiators, and methods for amplifying signal resulting from recognition events, thereby enhancing the detection of those recognition events. Methods include amplification achieved through polymerization using a nanoparticle initiator conjugated recognition element that is not consumed during the reaction. The polymer formed as a result of the absorption of light by the nanoparticle initiator and introduction of reactive species into a surrounding polymerizable monomer solution occurs in a spatially-limited region directly surrounding the nanoparticle initiator and is indicative of the recognition event(s). In one embodiment, a semiconductor quantum dot nanoparticle initiator is utilized. In another embodiment, a metal nanoparticle is utilized. In another embodiment, the signal is detected without instrumentation. In yet another embodiment, the signal is detected via a transmission-based instrument which captures an image of the formed polymer.